McClure J
J Pathol. 1980 May;131(1):21-33. doi: 10.1002/path.1711310103.
The subcutaneous injection of small quantities of LaCl3 and CeCl3 was followed by calcification of the dorsal fascia in mice. The calcification reaction was shown by separate positive histochemical tests for calcium ions (the chloranilic acid reaction) and phosphate (the von Kossa reaction). The injection of cadmium chloride and stannous salts caused damage to and a positive von Kossa reaction in the fibres of the panniculus carnosus muscle. The significance and specificity of the histochemical tests was determined by X-ray microprobe analysis of tissues injected with the above salts and other salts known to cause either combined chloranilic acid and von Kossa positive reactions or isolated von Kossa positive reactions in mouse skin. The pathogenesis of the reactions produced by LaCl3, CeCl3 and KMnO4 was studied by applying histochemical tests to tissues recovered at timed intervals after injection. The reactions produced by LaCl3 and CeCl3 were similar and occurred in the dorsal fascia with positive VK reactions occurring first after 18 followed by positive CA reactions after 24 hr. The distribution of these reactions suggested a haematogenous origin for the constituent ions. The injection of KMnO4 in a much higher dose caused damage to the skin and particularly the fibres of the panniculus carnosus muscle. In contrast to the other salts, the calcific reaction occurred in association with these fibres, showing equally positive von Kossa and chloranilic acid reactions from the early stages.
给小鼠皮下注射少量的氯化镧和氯化铈后，其背部筋膜出现钙化。通过分别针对钙离子（氯冉酸反应）和磷酸盐（冯·科萨反应）的阳性组织化学试验显示了钙化反应。注射氯化镉和亚锡盐会对腹直肌纤维造成损伤，并出现阳性冯·科萨反应。通过对注射上述盐类及其他已知会在小鼠皮肤中引起氯冉酸和冯·科萨联合阳性反应或单独冯·科萨阳性反应的盐类的组织进行X射线微探针分析，确定了组织化学试验的意义和特异性。通过对注射后不同时间间隔回收的组织进行组织化学试验，研究了氯化镧、氯化铈和高锰酸钾产生的反应的发病机制。氯化镧和氯化铈产生的反应相似，发生在背部筋膜，18小时后首先出现阳性VK反应，24小时后出现阳性CA反应。这些反应的分布表明其组成离子起源于血源性。注射高得多剂量的高锰酸钾会对皮肤尤其是腹直肌纤维造成损伤。与其他盐类不同，钙化反应与这些纤维相关，从早期阶段就显示出同样阳性的冯·科萨反应和氯冉酸反应。
