Henion J D, Maylin G A
J Anal Toxicol. 1980 Jul-Aug;4(4):185-91. doi: 10.1093/jat/4.4.185.
A sensitive, quantitative method has been developed for the determination of hydrochlorothiazide in equine plasma and urine. Thin-layer chromatography is used to screen for the presence of the drug in unknown samples. The TLC screening methods described provide minimum detection limits of 50 ng/mL in plasma and 25 ng/mL in urine. A silica micro chromatography column is used to clean up ethyl acetate extracts for HPLC analysis and mass spectral confirmation. An internal standard, trichloromethiazide, is used to derive quantitative data at concentrations as low as 25 ng/mL for plasma disappearance curves and urinary excretion rates.
已开发出一种灵敏的定量方法，用于测定马血浆和尿液中的氢氯噻嗪。采用薄层色谱法对未知样品中的药物进行筛查。所描述的薄层色谱筛查方法在血浆中的最低检测限为50 ng/mL，在尿液中的最低检测限为25 ng/mL。使用硅胶微色谱柱对乙酸乙酯提取物进行净化，以用于高效液相色谱分析和质谱确证。使用内标物三氯噻嗪，在血浆消除曲线和尿排泄率低至25 ng/mL的浓度下获取定量数据。
