Goodfellow P N, Tippett P
Nature. 1981 Jan 29;289(5796):404-5. doi: 10.1038/289404a0.
A monoclonal antibody, 12E7, raised against lymphocytes from a patient with a T-cell acute lymphocytic leukaemia reacts strongly with cortical thymocytes and, to a lesser extent, with other human cells. The antigen defined by 12E7 is not expressed on mouse or hamster cells; this species specificity allowed us to investigate the genetics of the expression of the 12E7 antigen using human--rodent somatic-cell hybrids. Hybrid cells which contain the human X chromosome as the only human genetic contribution react with 12E7. The X-linkage of the gene, or genes, controlling 12E7 antigen expression and the fact that 12E7 antigen is also expressed on red blood cells led us to investigate the possibility of a relationship with the X-linked Xg blood group system. Although the 12E7 and Xga antigens seem to be different antigenic determinants specified by different loci, we report here a sex-limited effect involving the Xg phenotype on the expression of 12E7 antigen.
一种针对一名T细胞急性淋巴细胞白血病患者淋巴细胞产生的单克隆抗体12E7，与皮质胸腺细胞强烈反应，对其他人类细胞的反应程度较小。12E7所定义的抗原在小鼠或仓鼠细胞上不表达；这种物种特异性使我们能够利用人-啮齿类体细胞杂种研究12E7抗原表达的遗传学。仅含有人X染色体作为唯一人类遗传成分的杂种细胞与12E7发生反应。控制12E7抗原表达的一个或多个基因的X连锁以及12E7抗原也在红细胞上表达这一事实，促使我们研究其与X连锁的Xg血型系统之间存在关联的可能性。尽管12E7和Xga抗原似乎是由不同基因座指定的不同抗原决定簇，但我们在此报告一种涉及Xg表型对12E7抗原表达的性别限制效应。
