Valenzuela P, Quiroga M, Zaldivar J, Rutter W J, Kirschner M W, Cleveland D W
Nature. 1981 Feb 19;289(5799):650-5. doi: 10.1038/289650a0.
Most of the mRNA sequences coding for alpha and beta tubulin in embryonic chick brain have been determined by sequencing of cloned cDNA copies of these mRNA copies of these mRNAs. From a 1,682-base pair cDNA sequence we have deduced the entire protein sequence for beta tubulin. For alpha tubulin, all but about 38 N-terminal amino acids have been deduced from the cDNA sequence. Although tyrosine has previously been shown to be post-translationally added to the C-terminus of alpha tubulin by a specific ligase, we conclude that the primary post-translational even must be the removal, not the addition of tyrosine because a terminal tyrosine is encoded by the mRNA.
通过对胚胎期鸡脑α和β微管蛋白的mRNA克隆cDNA拷贝进行测序，已确定了编码这些微管蛋白的大部分mRNA序列。从一个1682个碱基对的cDNA序列中，我们推导了β微管蛋白的完整蛋白质序列。对于α微管蛋白，除了大约38个N端氨基酸外，其余部分已从cDNA序列中推导出来。尽管先前已证明酪氨酸是通过一种特定的连接酶在翻译后添加到α微管蛋白的C端，但我们得出结论，翻译后的主要事件必定是酪氨酸的去除，而非添加，因为mRNA编码了一个末端酪氨酸。
