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大鼠脑发育过程中三种β-微管蛋白mRNA的调控

Regulation of three beta-tubulin mRNAs during rat brain development.

作者信息

Ginzburg I, Teichman A, Dodemont H J, Behar L, Littauer U Z

出版信息

EMBO J. 1985 Dec 30;4(13B):3667-73. doi: 10.1002/j.1460-2075.1985.tb04133.x.

DOI:10.1002/j.1460-2075.1985.tb04133.x
PMID:2868892
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554716/
Abstract

The nucleotide sequence of a complete rat brain beta-tubulin T beta 15 has been determined from three overlapping cDNA clones. The overall length of the T beta 15 sequence is 1589 bp and shows between 84.5% and 88.6% homology within the coding region as compared with chick and human beta-tubulin sequences. On the other hand, the 3'-non-coding region is highly divergent. Comparison of the derived amino acid sequences from different species demonstrates that the amino acid changes are not randomly distributed, but rather there are several conserved and two highly variable regions common to beta-tubulin polypeptides from various sources. The T beta 15 sequence encodes a dominant neuronal 1.8-kb beta-tubulin mRNA species. Two other minor beta-tubulin mRNA species of 2.6 and 2.9 kb are present in rat brain. By using two synthetic oligonucleotide probes complementary to the carboxyl-terminal divergent region and to the amino-terminal conserved region, we have shown that the three mRNAs are distinct species, which are developmentally regulated. The level of the 1.8-kb mRNA species increases till the age of 12 days thereafter its level decreases. The 2.9-kb mRNA is an early neuronal mRNA species, while the 2.6-kb mRNA is a late neuronal species which is detected at 30 days of rat brain development. The data illustrate that there is a differential expression of the beta-tubulin multigene family during rat brain development which may suggest different functions for the various beta-tubulin isotopes.

摘要

已从三个重叠的cDNA克隆中确定了完整的大鼠脑β-微管蛋白Tβ15的核苷酸序列。Tβ15序列的总长度为1589 bp，与鸡和人β-微管蛋白序列相比，其编码区域内的同源性在84.5%至88.6%之间。另一方面，3'-非编码区域高度不同。对不同物种推导的氨基酸序列进行比较表明，氨基酸变化并非随机分布，而是来自各种来源的β-微管蛋白多肽有几个保守区域和两个高度可变区域。Tβ15序列编码一种占主导地位的神经元1.8 kb的β-微管蛋白mRNA种类。大鼠脑中还存在另外两种较小的2.6 kb和2.9 kb的β-微管蛋白mRNA种类。通过使用与羧基末端不同区域和氨基末端保守区域互补的两种合成寡核苷酸探针，我们表明这三种mRNA是不同的种类，且受到发育调控。1.8 kb mRNA种类的水平在12天龄之前增加，此后其水平下降。2.9 kb mRNA是一种早期神经元mRNA种类，而2.6 kb mRNA是一种晚期神经元种类，在大鼠脑发育30天时可检测到。数据表明，在大鼠脑发育过程中β-微管蛋白多基因家族存在差异表达，这可能暗示各种β-微管蛋白异构体具有不同功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbe8/554716/7a3411e30a2f/emboj00279-0037-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbe8/554716/69d36853c686/emboj00279-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbe8/554716/9e609fc5dc8f/emboj00279-0036-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbe8/554716/3bce0d3b673a/emboj00279-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbe8/554716/7a3411e30a2f/emboj00279-0037-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbe8/554716/69d36853c686/emboj00279-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbe8/554716/9e609fc5dc8f/emboj00279-0036-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbe8/554716/3bce0d3b673a/emboj00279-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbe8/554716/7a3411e30a2f/emboj00279-0037-b.jpg

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