Terblanche H M, Labuschagne J M
Onderstepoort J Vet Res. 1980 Sep;47(3):179-85.
The development of a practical competitive protein-binding assay for plasma progesterone in cattle is described. With an intra-assay coefficient of variation of 5,46% and an interassay coefficient of variation of 14,25%, the method is sufficiently accurate and sensitive for practical purposes, and for use in routines and surveys. The statistical level of sensitivity was found to be in the region of 0,25 ng/ml based on the confidence limits of zero dose and 0,25 ng/ml, with the practical sensitivity level at 0,50 ng/ml. Method and reagent blanks were found to be negligible. The specificity of the assay is based entirely on the partial specificity of the petroleum ether used for the extraction of progesterone (87,5% extraction, n = 141).
本文描述了一种用于牛血浆孕酮的实用竞争性蛋白结合测定法的开发。该方法的批内变异系数为5.46%，批间变异系数为14.25%，对于实际应用以及常规检测和调查而言，其准确性和灵敏度足够。基于零剂量和0.25 ng/ml的置信限，统计灵敏度水平在0.25 ng/ml左右，实际灵敏度水平为0.50 ng/ml。发现方法空白和试剂空白可忽略不计。该测定法的特异性完全基于用于提取孕酮的石油醚的部分特异性（提取率87.5%，n = 141）。
