Pilotti S, Carbone A, Lombardi L, Tavolato C, Rilke F
Tumori. 1978 Oct 31;64(5):535-47. doi: 10.1177/030089167806400512.
The investigation was carried out on blood smears, bone marrow aspirates, one lymph node biopsy, and the surgically removed spleen of a 53-year-old man with hairy cell leukemia. In the blood smears stained with May-Grünwald-Giemsa, 60 to 70% of the hairy cells contained tubular inclusions that corresponded to the ribosome-lamella complexes demonstrated at electron microscopy. In blood smears, imprints and cryostatic sections of the lymph node and of the spleen, hairy cells revealed tartrate-resistant acid phosphatase, beta-glucuronidase and adenosine-triphosphatase activity. In the spleen neutral esterase and alkaline phosphatase demonstrated the numerical increase of the histiocytes, which ultrastructurally displayed phagocytic activity. The presence in the spleen of pseudosinuses lined by hairy cells was confirmed by electron microscopy as well as by cytoenzymology.
对一名患有毛细胞白血病的53岁男性的血涂片、骨髓穿刺物、一次淋巴结活检以及手术切除的脾脏进行了研究。在用May-Grünwald-Giemsa染色的血涂片中，60%至70%的毛细胞含有管状包涵体，这些包涵体与电子显微镜下显示的核糖体-板层复合体相对应。在淋巴结和脾脏的血涂片、印片及冷冻切片中，毛细胞显示出抗酒石酸酸性磷酸酶、β-葡萄糖醛酸酶和三磷酸腺苷酶活性。在脾脏中，中性酯酶和碱性磷酸酶显示组织细胞数量增加，超微结构显示其具有吞噬活性。通过电子显微镜和细胞酶学证实脾脏中存在由毛细胞衬里的假血窦。
