淋巴因子。VI. 人及其他异种血清中抑制豚鼠巨噬细胞迁移的因子。

Lymphokines. VI. Factors in human and other heterologous sera inhibiting the migration of guinea pig macrophages.

作者信息

Baer R, Vogt K, Lazary S, Geczy C, de Weck A L

出版信息

Z Immunitatsforsch Immunobiol. 1978 Dec;155(2):130-43.

Abstract

Different heterologous sera including human, rabbit, bovine and fetal calf serum (FCS) showed a strong inhibiting effect on the migration of guinea pig peritoneal macrophages (GPPM), compared to the migration of GPPM in homologous guinea pig serum. The inhibiting effect of these sera on the migration of horse monocytes on the other hand was much less marked. Fractionation of human and rabbit serum showed the 4 S fraction to be most inhibitory on GPPM migration. The migration inhibiting effect of heterologous sera on GPPM was prevented by addition of homologous (GP) serum, by absorption of the sera by various guinea pig cells, by heating at 56 degrees and by addition of alpha-L-fucose. Human sera were found to be strongly cytotoxic for 51Cr-labelled guinea pig erythrocytes and lymphocytes, but not for horse lymphocytes. Upon absorption with guinea pig erythrocytes, lymphocytes and kidney cells, the cytotoxicity of the human sera was strongly reduced. Accordingly, the role of heterophilic antibodies and/or of heat-labile MIF-like factors in heterologous sera has to be considered in al macrophage migration experiments where heterologous sera are being used. These factors may differ from the heat-stable MIF activity generated upon antigen- or mitogen-induced stimulation of cultured lymphocytes.

摘要

与豚鼠腹腔巨噬细胞（GPPM）在同源豚鼠血清中的迁移相比，包括人、兔、牛和胎牛血清（FCS）在内的不同异种血清对GPPM的迁移显示出强烈的抑制作用。另一方面，这些血清对马单核细胞迁移的抑制作用则不太明显。人血清和兔血清的分级分离显示，4S级分对GPPM迁移的抑制作用最强。通过添加同源（GP）血清、用各种豚鼠细胞吸收血清、在56℃加热以及添加α-L-岩藻糖，可以阻止异种血清对GPPM的迁移抑制作用。发现人血清对51Cr标记的豚鼠红细胞和淋巴细胞具有强烈的细胞毒性，但对马淋巴细胞则无此作用。用人红细胞、淋巴细胞和肾细胞吸收后，人血清的细胞毒性大大降低。因此，在所有使用异种血清的巨噬细胞迁移实验中，都必须考虑异种血清中嗜异性抗体和/或热不稳定MIF样因子的作用。这些因子可能与抗原或丝裂原诱导培养淋巴细胞刺激产生的热稳定MIF活性不同。