Evermann J F, Baumgartener L, Ott R L, Davis E V, McKeirnan A J
Vet Pathol. 1981 Mar;18(2):256-65. doi: 10.1177/030098588101800214.
A virus isolated in cell culture from the spleen of a cat with feline infectious peritonitis was identified by physicochemical, morphological and antigenic criteria as a coronavirus. The feline infectious peritonitis virus was compared in vitro with canine coronavirus, a reported enteric pathogen of dogs. The feline isolate was characterized, by chloroform sensitivity and resistance to 5-iododeoxyuridine, respectively, as containing essential lipid and an RNA genome. Other traits of the isolate included resistance to acidic conditions, heat lability, and resistance to trypsin. Electron microscopy showed viral particles with a structure consistent with that of the prototype of the coronavirus group, infectious bronchitis virus. Indirect immunofluorescence with canine coronavirus monospecific antiserum showed the viral isolate to be antigenically related to canine coronavirus. Specific-pathogen-free cats inoculated by various routes with cell-culture-propagated virus had both clinical symptoms and lesions consistent with feline infectious peritonitis.
从患有猫传染性腹膜炎的猫脾脏中分离出的一种病毒，通过物理化学、形态学和抗原标准被鉴定为冠状病毒。将猫传染性腹膜炎病毒在体外与犬冠状病毒进行了比较，犬冠状病毒是一种已报道的犬肠道病原体。该猫分离株分别通过对氯仿敏感和对5-碘脱氧尿苷耐药，被鉴定为含有必需脂质和RNA基因组。该分离株的其他特性包括耐酸性、热不稳定性和对胰蛋白酶的抗性。电子显微镜显示病毒颗粒的结构与冠状病毒组原型传染性支气管炎病毒一致。用犬冠状病毒单特异性抗血清进行的间接免疫荧光显示，该病毒分离株与犬冠状病毒有抗原相关性。通过各种途径接种细胞培养繁殖病毒的无特定病原体猫出现了与猫传染性腹膜炎一致的临床症状和病变。
