猫传染性腹膜炎病毒 DF-2 株的分子特征及 ORF3abc 对病毒细胞嗜性的作用研究。
Molecular characterization of feline infectious peritonitis virus strain DF-2 and studies of the role of ORF3abc in viral cell tropism.
机构信息
Department of Virology, Agricultural Office Veterinary Diagnostic Directorate, Budapest, Hungary.
出版信息
J Virol. 2012 Jun;86(11):6258-67. doi: 10.1128/JVI.00189-12. Epub 2012 Mar 21.
Abstract
The full-length genome of the highly lethal feline infectious peritonitis virus (FIPV) strain DF-2 was sequenced and cloned into a bacterial artificial chromosome (BAC) to study the role of ORF3abc in the FIPV-feline enteric coronavirus (FECV) transition. The reverse genetic system allowed the replacement of the truncated ORF3abc of the original FIPV DF-2 genome with the intact ORF3abc of the canine coronavirus (CCoV) reference strain Elmo/02. The in vitro replication kinetics of these two viruses was studied in CrFK and FCWF-4 cell lines, as well as in feline peripheral blood monocytes. Both viruses showed similar replication kinetics in established cell lines. However, the strain with a full-length ORF3 showed markedly lower replication of more than 2 log(10) titers in feline peripheral blood monocytes. Our results suggest that the truncated ORF3abc plays an important role in the efficient macrophage/monocyte tropism of type II FIPV.
摘要
高度致死性猫传染性腹膜炎病毒（FIPV）DF-2 全长基因组被测序并克隆到细菌人工染色体（BAC）中，以研究 ORF3abc 在 FIPV-猫传染性肠道冠状病毒（FECV）转变中的作用。该反向遗传学系统允许用犬冠状病毒（CCoV）参考株 Elmo/02 的完整 ORF3abc 替换原始 FIPV DF-2 基因组中截短的 ORF3abc。在 CrFK 和 FCWF-4 细胞系以及猫外周血单核细胞中研究了这两种病毒的体外复制动力学。这两种病毒在已建立的细胞系中显示出相似的复制动力学。然而，全长 ORF3 的病毒在猫外周血单核细胞中的复制明显低 2 个对数以上，滴度超过 2 个对数。我们的结果表明，截短的 ORF3abc 在 II 型 FIPV 中对巨噬细胞/单核细胞的高效嗜性中发挥重要作用。
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