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[使用超滤法获取无脂蛋白血清]

[Use of ultrafiltration to obtain serum devoid of lipoproteins].

作者信息

Titov V N, Chvileva O P, Cherniad'eva I F

出版信息

Vopr Med Khim. 1981 Jan-Feb;27(1):83-7.

PMID:7467209
Abstract

As compared with extraction of lipids using organic solvents or preparative ultracentrifugation a proposed procedure of ultrafiltration enabled to obtain rapidly an appropriate amounts of blood serum free of lipids and apoproteins, which, except for high molecular components impeded by membrane, is similar to the native blood serum. Blood serum free of both lipids and apoproteins permits of best interpretation of the data obtained in study of sterol synthesis in mononuclear leukocytes.

摘要

与使用有机溶剂提取脂质或制备性超速离心相比,所提出的超滤程序能够快速获得适量不含脂质和载脂蛋白的血清,除了被膜阻碍的高分子成分外,该血清与天然血清相似。不含脂质和载脂蛋白的血清有助于对单核白细胞中甾醇合成研究获得的数据进行最佳解读。

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