Goldstein A S, Hubbard M C, Barry J M
Am J Clin Pathol. 1981 Feb;75(2):221-4. doi: 10.1093/ajcp/75.2.221.
Freezing lymphocytes in microtest plates instead of vials for the purpose of screening sera for HLA or other cytotoxic antibodies offers the same technical advantages as freezing sera in microtest plates for tissue typing. Plate-loading errors and cell wastage are minimized, and time required for serum screening is greatly reduced. A method for freezing lymphocytes in large numbers of microtest plates for use in serum screening that is simple, reproducible, and inexpensive is described. Cryoprotected lymphocytes dispensed into microtest plates are placed on a metal table that is lowered onto a tray containing 1 cm of liquid nitrogen in a Styrofoam box. The amount of liquid nitrogen in the tray and the height of the metal table above the tray determine the freezing rate of the lymphocytes. Over 100 microtest plates can be frozen per cycle with total cooling uniformity. The materials required for this freezing device can be assembled for less than $400. Commercial freezing units typically cost over $4,000.
为筛选血清中的 HLA 或其他细胞毒性抗体而将淋巴细胞冻存于微量检测板而非冻存管中，与为组织分型而将血清冻存于微量检测板具有相同的技术优势。板加载误差和细胞浪费降至最低，血清筛选所需时间大幅减少。本文描述了一种用于血清筛选的在大量微量检测板中冻存淋巴细胞的方法，该方法简单、可重复且成本低廉。分装到微量检测板中的经冷冻保护的淋巴细胞置于金属台上，该金属台下降至一个置于聚苯乙烯泡沫塑料盒中装有 1 厘米液氮的托盘上。托盘中液氮的量以及金属台高于托盘的高度决定了淋巴细胞的冷冻速率。每个循环可冷冻 100 多个微量检测板，且具有完全的冷却均匀性。这种冷冻装置所需材料的组装成本不到 400 美元。而商用冷冻设备通常成本超过 4000 美元。
