一种将淋巴细胞板冷冻并储存在托盘中的简单方法。

A simple procedure for freezing and storing lymphocyte panels in trays.

作者信息

Barocci S, Costantini M, Kunkl A, Manca F

出版信息

J Immunol Methods. 1984 Apr 13;69(1):17-21. doi: 10.1016/0022-1759(84)90272-2.

Abstract

Lymphocytes from different donors may be frozen in Terasaki trays by a simple procedure. This method provides the tissue typing laboratory with a readily available panel that can be routinely used to test for lymphocytotoxic antibodies sera from patients waiting for kidney transplant, transplanted patients and polytransfused individuals. The advantage of this procedure is that expensive programmed freezing apparatus is not required.

摘要

通过一个简单的程序，可将来自不同供体的淋巴细胞冻存在特拉西基培养板中。这种方法为组织配型实验室提供了一个随时可用的细胞面板，可常规用于检测等待肾移植患者、移植患者和多次输血个体血清中的淋巴细胞毒性抗体。该程序的优点是不需要昂贵的程序冷冻设备。