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通过聚丙烯酰胺平板凝胶电泳对“衣原体”纯化原体进行多肽成分分析(作者译)

[Polypeptidic composition analysis of "Chlamydia" purified elementary bodies by polyacrylamide slab gel electrophoresis (author's transl)].

作者信息

Lefebvre J F, Orfila J

出版信息

Ann Microbiol (Paris). 1980 Nov-Dec;131B(3):223-37.

PMID:7469253
Abstract

The study of the polypeptidic components of elementary bodies of various strains of Chlamydia might help in the classification of these bacteria. The use of a satisfactory method for the preparation of purified elementary bodies has permitted the electrophoretic analysis of their crude lysate on polyacrylamide gel in the presence of sodium dodecyl sulfate. Differences between the strain LB 1 C. trachomatis and the strains Loth and MP 1 C. psittaci were found.

摘要

对各种衣原体菌株原体的多肽成分进行研究可能有助于对这些细菌进行分类。采用一种令人满意的制备纯化原体的方法,使得能够在十二烷基硫酸钠存在的情况下,对其粗裂解物在聚丙烯酰胺凝胶上进行电泳分析。发现沙眼衣原体LB 1菌株与鹦鹉热衣原体Loth菌株和MP 1菌株之间存在差异。

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