山黧豆（草豌豆）幼苗中对称高亚精胺的酶促合成
Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings.
作者信息
Srivenugopal K S, Adiga P R
出版信息
Biochem J. 1980 Aug 15;190(2):461-4. doi: 10.1042/bj1900461.
Abstract
An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.
摘要
一种可催化腐胺和NAD⁺合成同型高亚精胺并伴随NH₃释放的酶，通过在蓝色琼脂糖凝胶上进行亲和层析，从山黧豆（香豌豆）幼苗中纯化了100倍。这种硫醇酶的表观分子量为75000，表现出米氏动力学，对腐胺的Km值为3.0mM。在檀香（檀香木）叶片的粗提物中也能检测到相同的酶活性，但其比活性比山黧豆幼苗中的高15倍。
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