The digestibilities of microbial and food proteins in the small intestine were studied in three sheep fitted with re-entrant cannulas in the proximal duodenum and terminal ileum. 2. The quantities of microbial and food proteins at the small intestine were varied by infusion of a microbial isolate or by dietary manipulation and the balance of amino acids along the small intestine was determined. 3. A mean value of 0.69 for the apparent digestibility and 0.86 for the true digestibility of total amino acids was obtained. 4. From the composition of digesta at the duodenum the daily flows of microbial and food proteins were estimated. Their true digestibilities in the small intestine were calculated by regression and found to be: microbial protein 0.87 and food protein 0.82. The mean endogenous loss of amino acids secreted into the small intestine was estimated to be 13.3 g/d.
