Deutsch J W, Kelly R B
Biochemistry. 1981 Jan 20;20(2):378-85. doi: 10.1021/bi00505a024.
Synaptic vesicles from the electric organ of the marine ray Narcine brasiliensis, purified to at least 90% homogeneity, were analyzed for the lipid and fatty acid content of their membranes. The major lipids (mol %) were phosphatidylcholine (32.3%), phosphatidylethanolamine (20.5%), phosphatidylserine (6.1%), sphingomyelin (3.0%), and cholesterol (33.3%), a composition which did not differ greatly from that of the parent electric organ. While the number of double bonds per fatty acid molecule was similar for both synaptic vesicle and whole electric organ phospholipids, the vesicles were highly enriched in docosahexenoic acid (22:6). Reaction with the amine labeling reagents isethionylacetimidate and trinitrobenzenesulfonic acid indicated that 40% of the phosphatidylserine and 60% of the phosphatidylethanolamine are present on the external (cytoplasmic) surface of the synaptic vesicle. These data on a natural fusing membrane have relevance to models of membrane fusion, which have been based largely on studies of in vitro fusion using synthetic membranes.
对从巴西电鳐（Narcine brasiliensis）的电器官中纯化至至少90%纯度的突触小泡的膜脂质和脂肪酸含量进行了分析。主要脂质（摩尔百分比）为磷脂酰胆碱（32.3%）、磷脂酰乙醇胺（20.5%）、磷脂酰丝氨酸（6.1%）、鞘磷脂（3.0%）和胆固醇（33.3%），其组成与母本电器官的组成差异不大。虽然突触小泡和整个电器官磷脂的每个脂肪酸分子的双键数量相似，但突触小泡中二十二碳六烯酸（22:6）高度富集。与胺标记试剂羟乙磺酰乙亚胺酯和三硝基苯磺酸的反应表明，40%的磷脂酰丝氨酸和60%的磷脂酰乙醇胺存在于突触小泡的外部（细胞质）表面。这些关于天然融合膜的数据与膜融合模型相关，膜融合模型很大程度上基于使用合成膜的体外融合研究。
