Inoue Y
Biochim Biophys Acta. 1981 Feb 12;634(2):309-20. doi: 10.1016/0005-2728(81)90149-3.
The intensity of the A band of thermoluminescence from isolated spinach chloroplasts were greatly dependent on the excitation temperature between -40 and -10 degrees C. This dependency was analyzed in relation to the S states in the oxygen-evolving system, and the following results were obtained. 1. The A band was charged only slightly by excitation at low temperature -65 degrees C) with continuous light, but when two flashes had been given before cooling the A band was charged efficiently by the low temperature excitation. 2. The efficiency of the double flash pre-illumination for enhancing the A band showed a steep temperature dependency which agreed with the temperature dependency of the photoconversion from the S2 to S3 state. 3. The A-band-enhancing effect was dependent on the number of flashes, and underwent a quadruple oscillation. 4. The enhancing effect decayed in darkness and the decay course agreed with that of the B1 band but not with that of the B2 band. From these results, the origin of positive charges for the A band was discussed.
分离的菠菜叶绿体热释光A带的强度在-40℃至-10℃的激发温度下高度依赖。结合放氧系统中的S态对这种依赖性进行了分析，得到以下结果。1. 在-65℃低温下用连续光照激发时，A带仅有轻微充电，但在冷却前给予两次闪光后，A带可被低温激发有效充电。2. 双闪光预照明增强A带的效率呈现出陡峭的温度依赖性，这与从S2态到S3态的光转换的温度依赖性一致。3. A带增强效应取决于闪光次数，并经历四重振荡。4. 增强效应在黑暗中衰减，衰减过程与B1带一致，但与B2带不同。基于这些结果，对A带正电荷的来源进行了讨论。
