Linz D N, Garcia V F, Arya G, Hug G, Tombragel E, Landrigan E, Chuck G, Tsoras M, Ryan M, Ziegler M M
Department of Surgery, Case Western Reserve University, Cincinnati, OH, USA.
J Pediatr Surg. 1995 Jul;30(7):959-65; discussion 966. doi: 10.1016/0022-3468(95)90321-6.
Increased oxidation of fat is an important host response to sepsis, and carnitine is essential for long-chain fatty acid oxidation. Because neonates have low levels of carnitine, their ability to respond to a septic insult may be impaired. The purpose of this study was to compare fatty acid and carnitine metabolism in septic weanling (60 to 85 g) and septic adult (285 to 310 g) rats. Sepsis was induced in weanling and adult male Sprague-Dawley rats by cecal ligation and puncture (CLP). The rats were killed 16 hours after CLP or sham operation, and serum glucose, lactate, beta-hydroxybutyrate, fatty acid, carnitine, liver fatty acid, and tissue carnitine levels were measured. The data suggest that during sepsis weanling rats may be more dependent on fatty acid oxidation than adult rats are, as evidenced by their elevated serum fatty acid and acylcarnitine levels, and relative hypoglycemia and hyperketonemia. In addition, although total serum carnitine levels were increased in both adult and weanling septic rats, tissue carnitine levels of weanling rats became significantly depleted during sepsis, unlike in adult rats. This study supports further investigation regarding the role of exogenous carnitine in newborn sepsis.
脂肪氧化增加是机体对脓毒症的一种重要反应，而肉碱对于长链脂肪酸氧化至关重要。由于新生儿肉碱水平较低，他们对脓毒症损伤的反应能力可能会受损。本研究的目的是比较脓毒症断奶幼鼠（60至85克）和脓毒症成年大鼠（285至310克）的脂肪酸和肉碱代谢情况。通过盲肠结扎和穿刺（CLP）诱导断奶幼鼠和成年雄性Sprague-Dawley大鼠发生脓毒症。在CLP或假手术后16小时处死大鼠，测量血清葡萄糖、乳酸、β-羟基丁酸、脂肪酸、肉碱、肝脏脂肪酸和组织肉碱水平。数据表明，在脓毒症期间，断奶幼鼠可能比成年大鼠更依赖脂肪酸氧化，这可从它们升高的血清脂肪酸和酰基肉碱水平以及相对低血糖和高酮血症得到证明。此外，虽然成年和断奶脓毒症大鼠的血清总肉碱水平均升高，但与成年大鼠不同，断奶幼鼠的组织肉碱水平在脓毒症期间显著降低。本研究支持进一步探讨外源性肉碱在新生儿脓毒症中的作用。
