Györfi A, Fazekas A, Irmes F, Rosivall L
Institute of Pathophysiology, Semmelweis University of Medicine, Budapest, Hungary.
J Periodontal Res. 1995 May;30(3):181-5. doi: 10.1111/j.1600-0765.1995.tb01271.x.
Neuropeptides, including substance P (SP) may play a role in neurogenic inflammation. Although SP-immunoreactive (SP-IR) axons are known to be present within the oral mucosa (OM) and salivary glands, the functional significance of SP in oral mucosa and sublingual salivary gland (SLG) is not fully understood. The present experiments were carried out to study the effects of SP infused into the left common carotid artery on vascular permeability in the OM and in the SLG of male rats. Vascular permeability was assessed on the basis of Evans Blue extravasation. Separate groups of animals received histamine (H1) receptor antagonist (chloropyramine, 10 mg kg-1 i.v.) or prostaglandin synthesis inhibitor (indomethacin, 4 mg kg-1 i.v.) prior to SP infusions. Infusion of SP in doses of 30 and 74 pmol min-1 increased the vascular permeability of OM by 162.3 +/- 16.3% (n = 8, p < 0.05) and 482.7 +/- 46.7% (n = 8, p < 0.001), respectively. SP in a dose of 15 pmol min-1 did not increase Evans Blue extravasation in OM (38.3 +/- 4.0 micrograms g-1, n = 8, compared to the control: 44.0 +/- 7.9 micrograms g-1, n = 8, NS).(ABSTRACT TRUNCATED AT 250 WORDS)
包括P物质（SP）在内的神经肽可能在神经源性炎症中发挥作用。尽管已知SP免疫反应性（SP-IR）轴突存在于口腔黏膜（OM）和唾液腺中，但SP在口腔黏膜和舌下唾液腺（SLG）中的功能意义尚未完全明确。本实验旨在研究向雄性大鼠左颈总动脉注入SP对OM和SLG血管通透性的影响。基于伊文思蓝外渗评估血管通透性。在注入SP之前，将动物分成不同组，分别给予组胺（H1）受体拮抗剂（氯吡胺，10毫克/千克静脉注射）或前列腺素合成抑制剂（吲哚美辛，4毫克/千克静脉注射）。以30和74皮摩尔/分钟的剂量注入SP，分别使OM的血管通透性增加了162.3±16.3%（n = 8，p < 0.05）和482.7±46.7%（n = 8，p < 0.001）。以15皮摩尔/分钟的剂量注入SP并未增加OM中的伊文思蓝外渗（38.3±4.0微克/克，n = 8，与对照组相比：44.0±7.9微克/克，n = 8，无显著性差异）。（摘要截短至250字）
