Györfi A, Fazekas A, Irmes F, Jakab G, Sütö T, Rosivall L
Department of Pathophysiology, Semmelweiss University of Medicine, Budapest, Hungary.
J Periodontal Res. 1993 May;28(3):191-6. doi: 10.1111/j.1600-0765.1993.tb01068.x.
In the present series of investigations we first studied the local effects of exogenous substance P (SP) on the hallmarks of neurogenic inflammation, i.e. vascular permeability and blood flow, in the oral mucosa of the rat. Pretreatment with capsaicin was shown to attenuate the symptoms of neurogenic inflammation; therefore, the distribution of nerve fibers displaying substance P-like immunoreactivity (SP-IR) in the mandibular mucosa was also assessed in control rats and in animals pretreated with capsaicin both neonatally and in adulthood using immunohistochemical techniques. The application of SP at a dose of 7.5 nmol resulted in an almost 70% increase of vascular permeability (NS) and the administration of a four-fold higher dose (30 nmol) produced about 150% increase in Evans blue extravasation compared with control values (p < 0.05). A similar increase (ca 146%) in vascular permeability was observed in response to 45 nmol SP solution (p < 0.05). While the 7.5 nmol SP-solution failed to affect blood flow, the 30 nmol SP significantly increased it by ca. 38% (p < 0.05). The administration of the 45 nmol SP solution resulted in a similar enhancement of blood flow (43%, p < 0.05). Capsaicin pretreatment performed either neonatally or in adulthood has reduced the number of SP-immunoreactive fibers in the oral mucosa. Our functional results suggest that SP may have a role in the experimentally-induced neurogenic inflammation of the oral mucosa in the rat. This is also supported by our finding that capsaicin pretreatment, known to decrease the number of SP-immunoreactive fibers in these tissues, reduced the symptoms of neurogenic inflammation.
在本系列研究中，我们首先研究了外源性P物质（SP）对大鼠口腔黏膜神经源性炎症特征，即血管通透性和血流量的局部影响。结果表明，辣椒素预处理可减轻神经源性炎症症状；因此，还使用免疫组织化学技术评估了对照大鼠以及新生期和成年期经辣椒素预处理的动物下颌黏膜中显示P物质样免疫反应性（SP-IR）的神经纤维分布。与对照值相比，给予7.5 nmol剂量的SP导致血管通透性增加近70%（无显著性差异），而给予四倍高剂量（30 nmol）则使伊文思蓝外渗增加约150%（p<0.05）。响应45 nmol SP溶液，观察到血管通透性有类似增加（约146%，p<0.05）。虽然7.5 nmol SP溶液未影响血流量，但30 nmol SP使其显著增加约38%（p<0.05）。给予45 nmol SP溶液导致血流量有类似增强（43%，p<0.05）。新生期或成年期进行的辣椒素预处理减少了口腔黏膜中SP免疫反应性纤维的数量。我们的功能研究结果表明，SP可能在实验诱导的大鼠口腔黏膜神经源性炎症中起作用。这也得到了我们的发现的支持，即已知可减少这些组织中SP免疫反应性纤维数量的辣椒素预处理减轻了神经源性炎症症状。
