Yotis W W, Macaluso F, Gopalsami C
Department of Microbiology, Loyola University of Chicago, Stritch School of Medicine, Maywood, Illinois 60153, USA.
J Basic Microbiol. 1995;35(4):255-68. doi: 10.1002/jobm.3620350411.
In this study the extraction and the immunochemical features of a lipopolysaccharide-like (LPSL) macromolecule of T. denticola strains 35405, 35404, 33521 and 11 were investigated. The yield of LPSL molecule ranged between 0.5-0.9% of the cell dry weight, it possessed Limulus amebocyte lysate clotting activity, and it contained glucosamine, phosphate, heptose, glucose, small amounts of KDO, myristic and beta hydroxy myristic acid. Sera obtained from healthy individuals (ADA type I) periodontitis, from 3-8 month old infants, or the mouse monoclonal antibody, diluted 1:2, against T. pallidum did not react with the LPSL antigens of T. denticola strains 35405, 35404, 33521, and 11. Sera from patients with ADA type III-IV periodontitis were reactive with two 8-14 kDa bands even at serum dilutions of 1:2000. Sera from patients with ADA type II periodontitis showed good antibody response to the 8-14 kDa band at a dilution of 1:50, but were weekly reactive, or nonreactive at serum dilutions of 1:200. This study indicates that extraction of a lipopolysaccharide-like macromolecule is feasible from the assay spirochetes, and this macromolecule may be used as an antigen for the diagnosis of ADA types II-IV periodontitis.
在本研究中，对齿垢密螺旋体菌株35405、35404、33521和11的一种脂多糖样（LPSL）大分子的提取及免疫化学特性进行了研究。LPSL分子的产量占细胞干重的0.5 - 0.9%，具有鲎试剂凝血活性，且含有葡糖胺、磷酸盐、庚糖、葡萄糖、少量的KDO、肉豆蔻酸和β - 羟基肉豆蔻酸。从健康个体（ADA I型）、牙周炎患者、3 - 8月龄婴儿或针对梅毒螺旋体的小鼠单克隆抗体（稀释至1:2）获得的血清，均不与齿垢密螺旋体菌株35405、35404、33521和11的LPSL抗原发生反应。ADA III - IV型牙周炎患者的血清即使在1:2000的血清稀释度下，也能与两条8 - 14 kDa的条带发生反应。ADA II型牙周炎患者的血清在1:50的稀释度下对8 - 14 kDa条带显示出良好的抗体反应，但在1:200的血清稀释度下反应较弱或无反应。本研究表明，从检测的螺旋体中提取脂多糖样大分子是可行的，且该大分子可用作诊断ADA II - IV型牙周炎的抗原。
