Chang D Y, Hsieh C Y, Chen R J, Lee S C, Huang S C
Department of Obstetrics and Gynecology, College of Medicine and Hospital, National Taiwan University, Taipei.
J Med Microbiol. 1995 Dec;43(6):430-5. doi: 10.1099/00222615-43-6-430.
An association between human papillomavirus (HPV) and cervical neoplasia has been widely reported and HPV DNA is commonly detected in cervical carcinoma tissues. However, estimates of the prevalence of HPV infection differs among various detection methods. Seventy cases of cervical carcinoma were screened for HPV 16 infection by Southern blot hybridisation (SBH) and nested polymerase chain reaction (PCR). According to SBH, the prevalences of HPV 16 DNA in stage I (n = 40) and stage II (n = 30) cervical carcinomas were 52.5 and 63.3%, respectively, and the overall prevalence was 57.1% (40 of 70). By nested PCR, the prevalences of HPV 16 infection in stage I and II cervical carcinomas were 87.5 and 93.3%, respectively, and the overall prevalence was 90.3%. The prevalence of HPV DNA detected by nested PCR was significantly greater than that detected by SBH. The combined concordance of positive and negative results between SBH and nested PCR was 61.4%. The discrepancy resulted mainly from 25 cases (35.7%) that were positive by PCR but negative by SBH. A small copy number of HPV DNA in the these 25 cases was documented by a semi-quantitative PCR method. The nested PCR was more sensitive than SBH and detected cases with low amounts of HPV DNA. The detection of HPV infection varied between these two prevailing detection methods and this should be kept in mind in assessing various epidemiological data concerning HPV infection.
人乳头瘤病毒（HPV）与宫颈肿瘤之间的关联已被广泛报道，并且在宫颈癌组织中普遍检测到HPV DNA。然而，HPV感染的患病率估计在各种检测方法之间有所不同。通过Southern印迹杂交（SBH）和巢式聚合酶链反应（PCR）对70例宫颈癌病例进行HPV 16感染筛查。根据SBH检测，I期（n = 40）和II期（n = 30）宫颈癌中HPV 16 DNA的患病率分别为52.5%和63.3%，总体患病率为57.1%（70例中的40例）。通过巢式PCR检测，I期和II期宫颈癌中HPV 16感染的患病率分别为87.5%和93.3%，总体患病率为90.3%。巢式PCR检测到的HPV DNA患病率显著高于SBH检测到的患病率。SBH和巢式PCR之间阳性和阴性结果的联合一致性为61.4%。差异主要源于25例（35.7%）PCR检测为阳性但SBH检测为阴性的病例。通过半定量PCR方法记录了这25例病例中少量的HPV DNA。巢式PCR比SBH更敏感，能够检测到HPV DNA含量低的病例。这两种主要检测方法对HPV感染的检测结果存在差异，在评估有关HPV感染的各种流行病学数据时应牢记这一点。
