Wu C H, Lee M F, Chang M C, Ho S C
Department of Medical Research, Taichung Veterans General Hospital, Taiwan, Republic of China.
Sex Transm Dis. 1994 Nov-Dec;21(6):309-14. doi: 10.1097/00007435-199411000-00003.
The association of human papillomavirus (HPV) infection with cervical carcinoma is well documented. The HPV types in cervical lesions of patients from Taiwan are analyzed by the polymerase chain reaction (PCR).
DNA was extracted from paraffin-embedded, formalin-fixed tissues using a sonication method. PCR was performed using type-specific primers for the presence of HPV types 6, 11, 16, 18, 31, and 33 DNA. Amplified product was subjected to gel electrophoresis and Southern blot hybridization analysis.
A total of 129 cervical lesions and normal cervical biopsies were examined. Histologic examination revealed a spectrum of lesions, which were classified as condyloma acuminata (AC), condyloma (CL), koilocytosis (KL), various grades of cervical intraepithelial neoplasia (CIN I, II, and III), carcinoma in situ (CIS), and invasive carcinoma (ICa). Of 114 cervical lesions, 65% (26 of 40) of AC; 61% (11 of 18) of CL; 20% (2 of 10) of KL; 25% (1 of 4) of CIN I; 69% (9 of 13) of CIN II; 80% (12 of 15) of CIN III; 83% (5 of 6) of CIS; and 100% (8 of 8) of ICa were positive for at least one type of HPV by the PCR. Among the 74 HPV-positive specimens, 19 (26%) were detected with multiple types. HPV DNA was detected in the cervical biopsies of 1 of 15 (6.7%) normal individuals.
Excluding AC, HPV 6 and/or 11 (HPV 6/11), HPV 16 and/or 18 (HPV 16/18), and HPV 31 and/or 33 (HPV 31/33) were detected in 40% (19 of 48), 71% (34 of 48), and 12% (6 of 48) of neoplastic lesions of patients from Taiwan respectively. These findings are compatible with those reported by others worldwide.
人乳头瘤病毒（HPV）感染与宫颈癌之间的关联已有充分记录。采用聚合酶链反应（PCR）分析台湾患者宫颈病变中的HPV类型。
采用超声处理法从石蜡包埋、福尔马林固定的组织中提取DNA。使用针对HPV 6、11、16、18、31和33型DNA存在情况的型特异性引物进行PCR。对扩增产物进行凝胶电泳和Southern印迹杂交分析。
共检查了129例宫颈病变和正常宫颈活检组织。组织学检查显示了一系列病变，分为尖锐湿疣（AC）、湿疣（CL）、挖空细胞（KL）、不同级别的宫颈上皮内瘤变（CIN I、II和III）、原位癌（CIS）和浸润癌（ICa）。在114例宫颈病变中，PCR检测显示，40%（40例中的26例）的AC；61%（18例中的11例）的CL；20%（10例中的2例）的KL；25%（4例中的1例）的CIN I；69%（13例中的9例）的CIN II；80%（15例中的12例）的CIN III；83%（6例中的5例）的CIS；以及100%（8例中的8例）的ICa至少有一种HPV类型呈阳性。在74例HPV阳性标本中，19例（26%）检测到多种类型。在15例正常个体中的1例（6.7%）宫颈活检组织中检测到HPV DNA。
排除AC后，在台湾患者的肿瘤病变中，分别有40%（48例中的19例）、71%（48例中的34例）和12%（48例中的6例）检测到HPV 6和/或11（HPV 6/11）、HPV 16和/或18（HPV 16/18）以及HPV 31和/或33（HPV 31/33）。这些发现与全球其他地区的报道一致。
