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通过随机序列寡核苷酸筛选确定腺相关病毒Rep68和Rep78的结合位点

Determination of adeno-associated virus Rep68 and Rep78 binding sites by random sequence oligonucleotide selection.

作者信息

Chiorini J A, Yang L, Safer B, Kotin R M

机构信息

Molecular Hematology Branch, NIH/NHLBI, Bethesda, MD 20892-1654, USA.

出版信息

J Virol. 1995 Nov;69(11):7334-8. doi: 10.1128/JVI.69.11.7334-7338.1995.

DOI:10.1128/JVI.69.11.7334-7338.1995
PMID:7474165
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC189665/
Abstract

To further define the canonical binding site for the P5-promoted Rep proteins of the adeno-associated virus, a modified random oligonucleotide selection procedure was performed, using purified recombinant Rep protein. These results may explain the effects of Rep on cellular gene expression.

摘要

为了进一步确定腺相关病毒P5促进的Rep蛋白的典型结合位点，利用纯化的重组Rep蛋白进行了改良的随机寡核苷酸筛选程序。这些结果可能解释Rep对细胞基因表达的影响。

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Determination of adeno-associated virus Rep68 and Rep78 binding sites by random sequence oligonucleotide selection.通过随机序列寡核苷酸筛选确定腺相关病毒Rep68和Rep78的结合位点
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2
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本文引用的文献

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Biologically active Rep proteins of adeno-associated virus type 2 produced as fusion proteins in Escherichia coli.作为融合蛋白在大肠杆菌中产生的2型腺相关病毒的生物活性Rep蛋白。
J Virol. 1994 Feb;68(2):797-804. doi: 10.1128/JVI.68.2.797-804.1994.
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Identification of linear DNA sequences that specifically bind the adeno-associated virus Rep protein.鉴定与腺相关病毒Rep蛋白特异性结合的线性DNA序列。
J Virol. 1994 Aug;68(8):4988-97. doi: 10.1128/JVI.68.8.4988-4997.1994.
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A key transcription factor for eukaryotic initiation factor-2 alpha is strongly homologous to developmental transcription factors and may link metabolic genes to cellular growth and development.真核起始因子-2α的关键转录因子与发育转录因子高度同源，可能将代谢基因与细胞生长和发育联系起来。
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Adeno-associated virus (AAV) Rep proteins mediate complex formation between AAV DNA and its integration site in human DNA.腺相关病毒（AAV）的Rep蛋白介导AAV DNA与人DNA中其整合位点之间形成复合物。
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5808-12. doi: 10.1073/pnas.91.13.5808.
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