Haanes E J, Thomsen D R, Martin S, Homa F L, Lowery D E
Animal Health Discovery Research and Molecular Biology, Upjohn Company, Kalamazoo, Michigan, USA.
J Virol. 1995 Nov;69(11):7375-9. doi: 10.1128/JVI.69.11.7375-7379.1995.
We determined the nucleotide sequence of a 3.5-kb region of the bovine herpesvirus 1 (BHV-1) genome which contained the complete BHV-1 homologs of the herpes simplex virus type 1 (HSV-1) UL26 and UL26.5 genes. In HSV-1, the UL26 and UL26.5 open reading frames encode scaffold proteins upon which viral capsids are assembled. The UL26-encoded protein is also a proteinase and specifically cleaves both itself and the UL26.5-encoded protein. The overall BHV-1-encoded amino acid sequence showed only 41% identity to the HSV-1 sequences and was most divergent in the regions defined to be involved in the scaffolding function. We substituted the proteins encoded by the BHV-1 homologs of the UL26 and UL26.5 open reading frames, expressed in baculovirus, for the corresponding HSV-1 proteins in an in vitro HSV-1 capsid assembly system. The proteins expressed from the BHV-1 UL26 and UL26.5 homologs facilitated the formation of hybrid type B capsids indistinguishable from those formed entirely with HSV-1-encoded proteins.
我们测定了牛疱疹病毒1型（BHV-1）基因组中一个3.5kb区域的核苷酸序列，该区域包含单纯疱疹病毒1型（HSV-1）UL26和UL26.5基因的完整BHV-1同源物。在HSV-1中，UL26和UL26.5开放阅读框编码支架蛋白，病毒衣壳在其上组装。UL26编码的蛋白也是一种蛋白酶，可特异性切割其自身以及UL26.5编码的蛋白。BHV-1编码的氨基酸序列与HSV-1序列的整体一致性仅为41%，并且在定义为参与支架功能的区域中差异最大。我们用杆状病毒表达的BHV-1 UL26和UL26.5开放阅读框同源物编码的蛋白，在体外HSV-1衣壳组装系统中替代相应的HSV-1蛋白。由BHV-1 UL26和UL26.5同源物表达的蛋白促进了杂种B型衣壳的形成，这些衣壳与完全由HSV-1编码的蛋白形成的衣壳无法区分。
