单纯疱疹病毒1型UL25、UL26和UL26.5基因的马立克氏病病毒2型同源基因的鉴定与序列分析。
Identification and sequence analysis of the Marek's disease virus serotype 2 homologous genes of the herpes simplex virus type 1 UL25, UL26 and UL26.5 genes.
作者信息
Kato K, Jang H K, Izumiya Y, Cai J S, Tsushima Y, Miyazawa T, Kai C, Mikami T
机构信息
Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo, Japan.
出版信息
J Vet Med Sci. 1999 Jul;61(7):787-93. doi: 10.1292/jvms.61.787.
We identified and determined the nucleotide sequence of Marek's disease virus serotype 2 (MDV2) UL25, UL26 and UL26.5 homologous genes of herpes simplex virus type 1 (HSV-1). The UL25, UL26 and UL26.5 genes of HSV-1 encode virion proteins (UL25 and UL26.5) and serine protease (UL26). The deduced amino acid sequences of the three proteins show a high degree of homology to counterparts of HSV-1. By northern blot analyses we found that four transcripts whose sizes are 4.9, 3.9, 2.0 and 1.3 kb are transcribed from the domains of MDV2 genome containing the three genes. This is the first report dealing with UL25, UL26 and UL26.5 homologues of HSV-1 in MDV serotypes.
我们鉴定并确定了马立克氏病病毒2型（MDV2）中与单纯疱疹病毒1型（HSV-1）UL25、UL26和UL26.5同源的基因的核苷酸序列。HSV-1的UL25、UL26和UL26.5基因编码病毒体蛋白（UL25和UL26.5）和丝氨酸蛋白酶（UL26）。这三种蛋白推导的氨基酸序列与HSV-1的对应蛋白显示出高度同源性。通过Northern印迹分析，我们发现从MDV2基因组中包含这三个基因的区域转录出了大小分别为4.9、3.9、2.0和1.3 kb的四种转录本。这是关于MDV血清型中HSV-1的UL25、UL26和UL26.5同源物的首次报道。
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