Tresćec A, Valinger Z, Smerdel S, Tomasić J
Institute of Immunology, Zagreb, Croatia.
Biologicals. 1993 Jun;21(2):163-8. doi: 10.1006/biol.1993.1069.
A simple and sensitive immunoradiometric assay (IRMA) for detection of foetal calf serum proteins in vaccine preparations has been developed. In this two-site sandwich assay the same preparation of polyclonal anti-FCS immunoglobulin (IgG) was used for coating the solid phase (as a capture antibody) and in a labelled form (I-125 labelled) for detection. The developed assay allows precise and accurate quantification of FCS proteins (down to 10 ng/ml) in vaccine preparations and has been used for screening of FCS residues (a) during production of measles vaccine, and (b) in various different commercial vaccine preparations. An enzyme-linked immunosorbent assay (ELISA) was also developed based on the same assay two-site sandwich principle, where anti-FCS was directly labelled with horse-radish peroxidase. ELISA was comparable in sensitivity and accuracy with IRMA.
已开发出一种简单灵敏的免疫放射分析（IRMA）方法，用于检测疫苗制剂中的胎牛血清蛋白。在这种双位点夹心分析中，使用相同的多克隆抗胎牛血清免疫球蛋白（IgG）制剂包被固相（作为捕获抗体），并以标记形式（碘-125标记）用于检测。所开发的分析方法能够精确、准确地定量疫苗制剂中的胎牛血清蛋白（低至10 ng/ml），并已用于（a）麻疹疫苗生产过程中以及（b）各种不同商业疫苗制剂中胎牛血清残留的筛查。还基于相同的双位点夹心原理开发了一种酶联免疫吸附测定（ELISA）方法，其中抗胎牛血清直接用辣根过氧化物酶标记。ELISA在灵敏度和准确性方面与IRMA相当。
