Hosogaya S, Kume S
Department of Clinical and Laboratory Medicine, Yamanashi Medical University.
Nihon Rinsho. 1995 Sep;53(9):2203-7.
In enzyme immunoassay, the catalytic activity is determined by photometric assay of the substrate, and in a broad sense, it also includes fluorometric and chemiluminescent assays. Since enzyme immunoassay excells in specificity and detectability, it is widely applied to clinical tests which analyze various specimens. Here, we describe some of enzyme amplification methods including cyclic reactions, avidin-biotin reactions and enhanced chemiluminescent enzyme immunoassays. These methods have resulted in highly sensitive immunoassays, the level of which ranges from a attomole (1 x 10(-18) mol) to a zeptomole (1 x 10(-21) mol).
在酶免疫测定中，催化活性通过对底物的光度测定来确定，从广义上讲，它还包括荧光测定和化学发光测定。由于酶免疫测定在特异性和可检测性方面表现出色，因此广泛应用于分析各种标本的临床试验。在此，我们描述一些酶放大方法，包括循环反应、抗生物素蛋白-生物素反应和增强化学发光酶免疫测定。这些方法已产生了高灵敏度的免疫测定，其检测水平范围从阿托摩尔（1×10⁻¹⁸摩尔）到zeptomole（1×10⁻²¹摩尔）。
