Komuro T, Mukoyama R, Mukoyama H
Department of Legal Medicine, Nihon University School of Dentistry.
Nihon Rinsho. 1995 Sep;53(9):2322-9.
Medico-legal identification of saliva stains and bloodstains was performed by an enzyme-linked immunosorbent assay (ELISA) using a horseradish peroxidase conjugate in combination with the use of monoclonal antibodies. Activity of alpha-amylase in the stains was measured for an identification of saliva using an anti-human amylase antibody, and secretory IgA was detected for a species identification using an anti-IgA antibody. ABO and Lewis blood group antigens were detected using anti-A, anti-B, anti-H, anti-Lea and anti-Leb antibodies. After the solubilization of ABH antigens in blood stains with octyl-beta-D-glucose solution, ABO typing has been performed using fully automated system composed of a unit for blood group reaction based on ELISA and a unit for reading of result based on photometric analysis.
采用酶联免疫吸附测定（ELISA），结合辣根过氧化物酶缀合物及单克隆抗体，对唾液斑和血斑进行法医学鉴定。使用抗人淀粉酶抗体测定斑迹中α-淀粉酶的活性以鉴定唾液，使用抗IgA抗体检测分泌型IgA以进行种属鉴定。使用抗A、抗B、抗H、抗Lea和抗Leb抗体检测ABO和Lewis血型抗原。用辛基-β-D-葡萄糖溶液溶解血斑中的ABH抗原后，使用由基于ELISA的血型反应单元和基于光度分析的结果读取单元组成的全自动系统进行ABO血型分型。
