Steube K G, Jadau A, Teepe D, Drexler H G
DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen), German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Leukemia. 1995 Nov;9(11):1841-6.
The expression of the proto-oncogene bcl-2 was examined in a panel of 75 continuous human leukemia-lymphoma cell lines originated from different hematopoietic cell types. The presence of the bcl-2 protein, as evidenced by Western blotting, and its mRNA, as determined by Northern blotting, were not restricted to cells with the chromosomal translocation t(14;18)(q32;q21), but were also detected in a large number of cell lines without t(14;18). The amount of the bcl-2 protein and mRNA in the cell lines with t(14;18) was in the same order of magnitude as in other bcl-2 expressing cell lines of the same lineage, but without the translocation. Bcl-2 was found in all types of hematopoietic cell lines which were assigned to the following lineages based on their phenotypical characteristics: pre-B, B, plasma, T, myeloid, monocytic, erythroid-megakaryocytic and Hodgkin's lymphoma derived cell lines. The levels of accumulated mRNA and protein corresponded fairly well in most of the cell lines examined. Our results suggest the notion that bcl-2 expression is widely present in hematopoietic cell lines without restriction to single lineages and, in fact, clearly independent of the chromosomal aberration t(14;18). It is conceivable that bcl-2 expression is a common feature in established hematopoietic cell lines and may contribute to their unlimited growth in vitro.
在一组源自不同造血细胞类型的75个人类白血病-淋巴瘤连续细胞系中检测了原癌基因bcl-2的表达。通过蛋白质印迹法证实存在bcl-2蛋白，通过Northern印迹法测定其mRNA，其表达不仅限于具有染色体易位t(14;18)(q32;q21)的细胞，在大量无t(14;18)的细胞系中也能检测到。有t(14;18)的细胞系中bcl-2蛋白和mRNA的量与同一谱系中其他表达bcl-2但无该易位的细胞系处于相同数量级。在根据表型特征归为以下谱系的所有类型造血细胞系中均发现了bcl-2：前B细胞、B细胞、浆细胞、T细胞、髓细胞、单核细胞、红系-巨核细胞系以及霍奇金淋巴瘤衍生细胞系。在所检测的大多数细胞系中，积累的mRNA水平和蛋白水平相当吻合。我们的结果表明，bcl-2表达广泛存在于造血细胞系中，不限于单一谱系，实际上明显独立于染色体畸变t(14;18)。可以想象，bcl-2表达是已建立的造血细胞系的一个共同特征，可能有助于它们在体外的无限生长。
