DaSilva N, Hu Z B, Ma W, Rosnet O, Birnbaum D, Drexler H G
Department of Human and Animal Cell Cultures, Braunschweig, Germany.
Leukemia. 1994 May;8(5):885-8.
The FLT3 gene encodes a protein that appears to function as a receptor for a hematopoietic growth factor; together with the KIT and FMS receptors, FLT3 belongs to the superfamily of receptors with tyrosine kinase activity. We examined the expression of FLT3 mRNA in 36 human leukemia-lymphoma cell lines using Northern blot analysis. FLT3 transcripts were found in seven of seven pre B-ALL cell lines (derived from cases with pre B-acute lymphoblastic leukemia or chronic myeloid leukemia in lymphoid blast crisis), and in one of six B-cell lines (namely in a cell line established from a hairy cell leukemia). FLT3 message was not detected in five T-cell, five myeloid, four monocytic, four erythroid and five megakaryocytic cell lines. Two major mRNA species were expressed differentially by positive cell lines. KIT mRNA expression was also investigated in the same panel of cell lines, but was found only in cell lines with erythroid and megakaryocytic features (and not in any of the FLT3-positive cell lines). The pattern of expression of FLT3 contrasts with the transcription of FMS and KIT and suggests that the FLT3 product may play a role primary in immature lymphoid cells.
FLT3基因编码一种蛋白质，该蛋白质似乎作为造血生长因子的受体发挥作用；FLT3与KIT和FMS受体一起，属于具有酪氨酸激酶活性的受体超家族。我们使用Northern印迹分析检测了36种人白血病淋巴瘤细胞系中FLT3 mRNA的表达。在7种前B-ALL细胞系（源自前B急性淋巴细胞白血病或慢性髓性白血病淋巴细胞母细胞危象病例）的所有细胞系中，以及6种B细胞系中的1种（即源自毛细胞白血病建立的细胞系）中发现了FLT3转录本。在5种T细胞系、5种髓细胞系、4种单核细胞系、4种红系细胞系和5种巨核细胞系中未检测到FLT3信息。两种主要的mRNA种类在阳性细胞系中差异表达。还在同一组细胞系中研究了KIT mRNA的表达，但仅在具有红系和巨核细胞特征的细胞系中发现（在任何FLT3阳性细胞系中均未发现）。FLT3的表达模式与FMS和KIT的转录形成对比，表明FLT3产物可能主要在未成熟淋巴细胞中发挥作用。
