Tsurusawa M, Aoyama M, Saeki K, Fujimoto T
Department of Pediatrics, Aichi Medical University, Japan.
Leukemia. 1995 Nov;9(11):1921-5.
Cell cycle kinetics of childhood acute leukemia were determined by the in vitro labeling of marrow blast cells with bromodeoxyuridine (BrdUrd) and subsequent flow cytometry of BrdUrd/DNA and Ki67/DNA in 18 patients with acute lymphocytic leukemia (ALL) and eight patients with acute nonlymphocytic leukemia (ANLL). The BrdUrd-labeling index (BrdUrd-LI) and the duration of S phase (Ts) were calculated from the slope of the regression line obtained by plotting the serial labeling indices against the labeling time. The Ts and potential doubling time (DTpot) of marrow leukemia cells varied from 6.1 to 34.3 h (median 14.3 h) and 1.1 to 20.7 days (median, 7.3 days), respectively. The duration of the total cell cycle time (Tc) which was determined by the Ki-67-derived growth fraction (Ki-67-GF) varied from 14.0 to 112.5 h (median 43.2 h). BrdUrd-LI, DTpot, Ki-67-GF and Tc were significantly correlated with the subtypes (early B-ALL, T/B- ALL and ANLL) of the disease. The median values of LI and GF were much lower in ANLL than in ALL. However, the low proliferative activity of ANLL was not accompanied by a prolonged duration of the total cell cycle time. The longest median duration of Tc was noted in early B-ALL (75.2 h) and the median Tc in ANLL (36.7 h) was close to that in T/B-ALL (34 h). Ts appeared to be rather independent of subtypes of the disease. These results show that there are distinct in vitro growth characteristics in relation to the subtypes of childhood acute leukemia.
通过用溴脱氧尿苷（BrdUrd）对骨髓原始细胞进行体外标记，并随后对18例急性淋巴细胞白血病（ALL）患者和8例急性非淋巴细胞白血病（ANLL）患者的BrdUrd/DNA和Ki67/DNA进行流式细胞术检测，确定了儿童急性白血病的细胞周期动力学。根据将连续标记指数与标记时间作图得到的回归线斜率计算BrdUrd标记指数（BrdUrd-LI）和S期持续时间（Ts）。骨髓白血病细胞的Ts和潜在倍增时间（DTpot）分别为6.1至34.3小时（中位数14.3小时）和1.1至20.7天（中位数7.3天）。由Ki-67衍生生长分数（Ki-67-GF）确定的总细胞周期时间（Tc）为14.0至112.5小时（中位数43.2小时）。BrdUrd-LI、DTpot、Ki-67-GF和Tc与该疾病的亚型（早期B-ALL、T/B-ALL和ANLL）显著相关。ANLL中LI和GF的中位数远低于ALL。然而，ANLL的低增殖活性并未伴随着总细胞周期时间的延长。早期B-ALL的Tc中位数最长（75.2小时），ANLL的Tc中位数（36.7小时）与T/B-ALL的相近（34小时）。Ts似乎与疾病亚型相当独立。这些结果表明，儿童急性白血病各亚型具有不同的体外生长特征。
