Ohno T, Oka K, Yamaguchi M, Kita K, Shirakawa S
Second Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
Leukemia. 1995 Nov;9(11):1935-9.
A panel of 14 anti-shared idiotype (Sid) monoclonal antibodies selected according to their high cross-reactivity to various lymphomas was immunohistologically tested for reactivity with seven reactive lymphoid tissue specimens and 227 B cell lymphoma specimens obtained from Japanese patients. In the reactive lymphoid tissues, the anti-Sld antibodies each reacted with a subpopulation of cells in the mantle zone and interfollicular areas; they rarely reacted with cells in the germinal center. In the B cell lymphomas, 13 anti-Sld antibodies reacted with a total of 78 of 186 (42%) specimens bearing immunoglobulin; none of the antibodies reacted with 41 specimens not bearing immunoglobulin. In mantle cell lymphomas (15/19, 79%) and extranodal small lymphocytic/non-mantle cell diffuse small cleaved lymphomas (11/15, 73%), the reactivity of the antibodies was high compared with that in the other lymphomas (52/152, 34%; P = 0.0002 and 0.004, respectively), including follicular lymphomas (11/42, 27%; P = 0.002 and 0.002, respectively). Since idiotypes are associated with the hypervariable regions and antigen-binding sites of immunoglobulin, these findings may reflect the differences in the regions/sites in each of these diseases.
根据对各种淋巴瘤具有高交叉反应性而选出的一组14种抗独特型（Sid）单克隆抗体，对来自日本患者的7份反应性淋巴组织标本和227份B细胞淋巴瘤标本进行了免疫组织化学检测，以检测其反应性。在反应性淋巴组织中，每种抗Sid抗体都与外套层区和滤泡间区的一部分细胞发生反应；它们很少与生发中心的细胞发生反应。在B细胞淋巴瘤中，13种抗Sid抗体与186份携带免疫球蛋白的标本中的78份（42%）发生反应；没有一种抗体与41份未携带免疫球蛋白的标本发生反应。在外套细胞淋巴瘤（15/19，79%）和结外小淋巴细胞/非外套细胞弥漫性小裂细胞淋巴瘤（11/15，73%）中，抗体的反应性高于其他淋巴瘤（52/152，34%；P分别为0.0002和0.004），包括滤泡性淋巴瘤（11/42，27%；P分别为0.002和0.002）。由于独特型与免疫球蛋白的高变区和抗原结合位点相关，这些发现可能反映了这些疾病中各自区域/位点的差异。
