Miller R A, Hart S, Samoszuk M, Coulter C, Brown S, Czerwinski D, Kelkenberg J, Royston I, Levy R
IDEC Pharmaceuticals Corporation, Mountain View, Calif 94043.
N Engl J Med. 1989 Sep 28;321(13):851-7. doi: 10.1056/NEJM198909283211302.
Each B-cell lymphoma expresses a surface immunoglobulin that contains unique antigenic determinants (idiotypes). We have produced 199 murine monoclonal antibodies reactive with the idiotypes isolated from 67 patients with follicular small-cleaved-cell lymphoma. These antiidiotype antibodies were analyzed for their ability to react with lymphoma cells from patients other than the one against which each antibody was made. Twenty of the 199 antiidiotype antibodies were reactive with lymphoma cells from more than one patient. Depending on the antibody, the frequency of idiotype sharing ranged from 0.6 to 6.2 percent of B-cell lymphoma tumors evaluated. Tumors could be grouped into distinct families on the basis of their reactivity with these antibodies. In the aggregate, the 20 antibodies reacted with a total of 49 of 150 B-cell lymphomas (33 percent), including 30 of 110 follicular small-cleaved-cell lymphomas (27 percent). Many of these shared idiotypes were expressed by more than one histopathological subtype of lymphoma. We conclude that a panel of antibodies reactive with shared idiotypes can be produced for patients with B-cell lymphoma, obviating the need to produce an antiidiotype antibody for each patient.
每个B细胞淋巴瘤都表达一种含有独特抗原决定簇（独特型）的表面免疫球蛋白。我们制备了199种与从67例滤泡性小裂细胞淋巴瘤患者分离出的独特型发生反应的鼠单克隆抗体。分析了这些抗独特型抗体与制备每种抗体所针对患者以外的其他患者的淋巴瘤细胞发生反应的能力。199种抗独特型抗体中有20种与不止一名患者的淋巴瘤细胞发生反应。根据抗体不同，所评估的B细胞淋巴瘤肿瘤中独特型共享的频率范围为0.6%至6.2%。根据肿瘤与这些抗体的反应性，可将肿瘤分为不同的家族。总体而言，这20种抗体与150例B细胞淋巴瘤中的49例（33%）发生反应，包括110例滤泡性小裂细胞淋巴瘤中的30例（27%）。许多这些共享独特型由不止一种淋巴瘤组织病理学亚型表达。我们得出结论，可为B细胞淋巴瘤患者制备一组与共享独特型发生反应的抗体，从而无需为每个患者制备抗独特型抗体。
