Bozhenok L N, Chernolovskaia E L, Gorozhankin A V, Baĭborodin S I, Bolobolova E U, Kiseleva E V, Kerkis A Iu, Kobets N D
Mol Biol (Mosk). 1995 Jul-Aug;29(4):862-70.
Reaction of (pdT)16 derivatives, bearing 4-(N-2-chloroethyl-N-methylamino)benzylphosphamide group on its 5' end and biotin on its 3' end with DNA in interphase nuclei and metaphase chromosomes has been investigated by fluorescence and electron microscopy. The result obtained evidence that in interphase nuclei DNA in active chromatin (nucleolus) is the most available for specific modification. In metaphase chromosomes the modified DNA regions are situated on the surface of chromosome.
对5'端带有4-(N-2-氯乙基-N-甲氨基)苄基磷酰胺基团且3'端带有生物素的(pdT)16衍生物与间期细胞核和中期染色体中的DNA的反应，已通过荧光显微镜和电子显微镜进行了研究。所得结果证明，在间期细胞核中，活性染色质（核仁）中的DNA最易于进行特异性修饰。在中期染色体中，修饰的DNA区域位于染色体表面。
