Isaacman D J, Zhang Y, Rydquist-White J, Wadowsky R M, Post J C, Ehrlich G D
Department of Pediatrics, University of Pittsburgh School of Medicine, PA 15213-2583, USA.
Mol Cell Probes. 1995 Jun;9(3):157-60. doi: 10.1006/mcpr.1995.0026.
A polymerase chain reaction (PCR) assay based on the penicillin-binding protein gene PBP2B identified the presence of DNA specific for Streptococcus pneumoniae in the serum and CSF of a patient with culture-proven bacteremia and meningitis. Positive signals were seen to dilutions of 1:125 and 1:390,625 for the blood and CSF specimens, respectively. Potential advantages of PCR over conventional culture include exquisite sensitivity, faster results and the ability to identify the organisms by the presence of species-specific DNA even in patients pretreated with antibiotics.
基于青霉素结合蛋白基因PBP2B的聚合酶链反应（PCR）检测，在一名经培养证实患有菌血症和脑膜炎的患者的血清和脑脊液中，鉴定出了肺炎链球菌特异性DNA的存在。血液和脑脊液标本分别稀释至1:125和1:390,625时仍可见阳性信号。与传统培养相比，PCR的潜在优势包括极高的灵敏度、更快的结果以及即使在接受过抗生素预处理的患者中，也能通过物种特异性DNA的存在来鉴定病原体。
