通过pbp2b聚合酶链反应-限制性片段长度多态性分析检测肺炎链球菌对青霉素的敏感性
Detection of penicillin susceptibility in Streptococcus pneumoniae by pbp2b PCR-restriction fragment length polymorphism analysis.
作者信息
O'Neill A M, Gillespie S H, Whiting G C
机构信息
Department of Medical Microbiology, Royal Free Hospital School of Medicine, London NW3 2PF, United Kingdom.
出版信息
J Clin Microbiol. 1999 Jan;37(1):157-60. doi: 10.1128/JCM.37.1.157-160.1999.
Abstract
A PCR-restriction fragment length polymorphism strategy directed against the pbp2b gene was evaluated for identification of penicillin susceptibility. A total of 106 United Kingdom (U.K.), 30 Danish, and 11 Papua New Guinean strains were tested. Of the U.K. strains, all the susceptible and all but one of the resistant isolates were correctly assigned. By using conventional definitions of "not resistant" and "not susceptible," the sensitivities were 97. 5 and 94.4%, the specificities were 100 and 98.9%, the positive predictive values were 100 and 94.4%, and the negative predictive values were 93.1 and 98.9%, respectively. This technique may allow susceptible (MIC, <0.1 mg/liter) and resistant (MIC, >1 mg/liter) isolates to be distinguished in a single PCR.
摘要
针对pbp2b基因的聚合酶链反应-限制性片段长度多态性策略用于评估青霉素敏感性。共检测了106株来自英国、30株来自丹麦以及11株来自巴布亚新几内亚的菌株。在英国菌株中，所有敏感菌株以及除1株外的所有耐药菌株均被正确分类。按照“不耐药”和“不敏感”的传统定义，敏感性分别为97.5%和94.4%，特异性分别为100%和98.9%，阳性预测值分别为100%和94.4%，阴性预测值分别为93.1%和98.9%。该技术可能使敏感（最低抑菌浓度，<0.1毫克/升）和耐药（最低抑菌浓度，>1毫克/升）菌株在一次聚合酶链反应中得以区分。
相似文献
1
Detection of penicillin susceptibility in Streptococcus pneumoniae by pbp2b PCR-restriction fragment length polymorphism analysis.通过pbp2b聚合酶链反应-限制性片段长度多态性分析检测肺炎链球菌对青霉素的敏感性
J Clin Microbiol. 1999 Jan;37(1):157-60. doi: 10.1128/JCM.37.1.157-160.1999.
2
Rapid screening for penicillin susceptibility of systemic pneumococcal isolates by restriction enzyme profiling of the pbp2B gene.通过pbp2B基因的限制性酶切图谱快速筛选系统性肺炎球菌分离株的青霉素敏感性
J Clin Microbiol. 1998 Aug;36(8):2359-62. doi: 10.1128/JCM.36.8.2359-2362.1998.
3
4
Unique variations of pbp2b sequences in penicillin-nonsusceptible Streptococcus pneumoniae isolates from Korea.来自韩国的青霉素不敏感肺炎链球菌分离株中pbp2b序列的独特变异
J Clin Microbiol. 2004 Apr;42(4):1746-50. doi: 10.1128/JCM.42.4.1746-1750.2004.
5
6
Rapid detection of penicillin-resistant Streptococcus pneumoniae in cerebrospinal fluid by a seminested-PCR strategy.采用半巢式聚合酶链反应策略快速检测脑脊液中耐青霉素肺炎链球菌
J Clin Microbiol. 1998 Feb;36(2):453-7. doi: 10.1128/JCM.36.2.453-457.1998.
7
Nasopharyngeal carriage of drug-resistant Streptococcus pneumoniae in children with acute otitis media evaluated by polymerase chain reaction-based genotyping of penicillin-binding proteins.通过基于聚合酶链反应的青霉素结合蛋白基因分型评估急性中耳炎患儿鼻咽部耐多药肺炎链球菌的携带情况。
Acta Otolaryngol. 2002 Jan;122(1):72-7. doi: 10.1080/00016480252775779.
8
Penicillin-resistant viridans streptococci have obtained altered penicillin-binding protein genes from penicillin-resistant strains of Streptococcus pneumoniae.耐青霉素的草绿色链球菌已从耐青霉素的肺炎链球菌菌株获得了改变的青霉素结合蛋白基因。
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5858-62. doi: 10.1073/pnas.87.15.5858.
9
Molecular characteristics of penicillin-binding protein genes of penicillin-nonsusceptible Streptococcus pneumoniae isolated in the Netherlands.荷兰分离出的对青霉素不敏感的肺炎链球菌青霉素结合蛋白基因的分子特征
Microb Drug Resist. 2001 Winter;7(4):323-34. doi: 10.1089/10766290152773338.
10
Application of a multiplex pbp2b and pbp2x PCR for prediction of penicillin resistance in Streptococcus pneumoniae.多重pbp2b和pbp2x聚合酶链反应在预测肺炎链球菌青霉素耐药性中的应用
J Antimicrob Chemother. 2004 May;53(5):890-1. doi: 10.1093/jac/dkh216. Epub 2004 Apr 8.
引用本文的文献
1
Simultaneous detection of Streptococcus pneumoniae, S. mitis, and S. oralis by a novel multiplex PCR assay targeting the gyrB gene.一种新型多重 PCR 检测方法，针对 gyrB 基因，同时检测肺炎链球菌、缓症链球菌和口腔链球菌。
J Clin Microbiol. 2013 Mar;51(3):835-40. doi: 10.1128/JCM.02920-12. Epub 2012 Dec 26.
2
Identification of a pheA gene associated with Streptococcus mitis by using suppression subtractive hybridization.利用抑制性消减杂交技术鉴定与变形链球菌相关的 pheA 基因。
Appl Environ Microbiol. 2012 Apr;78(8):3004-9. doi: 10.1128/AEM.07510-11. Epub 2012 Feb 3.
3
Evaluation of several biochemical and molecular techniques for identification of Streptococcus pneumoniae and Streptococcus pseudopneumoniae and their detection in respiratory samples.评价几种用于鉴定肺炎链球菌和咽峡炎链球菌及其在呼吸道样本中检测的生化和分子技术。
J Clin Microbiol. 2012 Apr;50(4):1171-7. doi: 10.1128/JCM.06609-11. Epub 2012 Jan 25.
4
Population structure of Streptococcus oralis.口腔链球菌的种群结构
Microbiology (Reading). 2009 Aug;155(Pt 8):2593-2602. doi: 10.1099/mic.0.027284-0. Epub 2009 May 7.
5
Duplex real-time PCR assay for detection of Streptococcus pneumoniae in clinical samples and determination of penicillin susceptibility.用于临床样本中肺炎链球菌检测及青霉素敏感性测定的双重实时聚合酶链反应检测法
J Clin Microbiol. 2008 Aug;46(8):2751-8. doi: 10.1128/JCM.02462-07. Epub 2008 Jun 18.
6
Discrimination of Streptococcus pneumoniae from viridans group streptococci by genomic subtractive hybridization.通过基因组消减杂交法区分肺炎链球菌与草绿色链球菌群。
J Clin Microbiol. 2005 Sep;43(9):4528-34. doi: 10.1128/JCM.43.9.4528-4534.2005.
7
Empyema: the use of broad range 16S rDNA PCR for pathogen detection.脓胸：使用广谱16S rDNA聚合酶链反应进行病原体检测。
Arch Dis Child. 2005 Jan;90(1):70-3. doi: 10.1136/adc.2003.042176.
8
Epidemiological aspects of antibiotic resistance in respiratory pathogens.呼吸道病原体抗生素耐药性的流行病学特征
Int J Antimicrob Agents. 2001 Dec;18(6):497-502. doi: 10.1016/s0924-8579(01)00455-1.
9
Molecular detection of antimicrobial resistance.抗菌药物耐药性的分子检测
Clin Microbiol Rev. 2001 Oct;14(4):836-71, table of contents. doi: 10.1128/CMR.14.4.836-871.2001.
10
Sensitive and specific method for rapid identification of Streptococcus pneumoniae using real-time fluorescence PCR.使用实时荧光PCR快速鉴定肺炎链球菌的灵敏且特异的方法。
J Clin Microbiol. 2001 Oct;39(10):3446-51. doi: 10.1128/JCM.39.10.3446-3451.2001.
本文引用的文献
1
The estimation of the bactericidal power of the blood.血液杀菌能力的评估。
J Hyg (Lond). 1938 Nov;38(6):732-49. doi: 10.1017/s002217240001158x.
2
Rapid detection of penicillin-resistant Streptococcus pneumoniae in cerebrospinal fluid by a seminested-PCR strategy.采用半巢式聚合酶链反应策略快速检测脑脊液中耐青霉素肺炎链球菌
J Clin Microbiol. 1998 Feb;36(2):453-7. doi: 10.1128/JCM.36.2.453-457.1998.
3
An outbreak of penicillin resistant Streptococcus pneumoniae investigated by a polymerase chain reaction based genotyping method.采用基于聚合酶链反应的基因分型方法对青霉素耐药肺炎链球菌的一次暴发进行调查。
J Clin Pathol. 1997 Oct;50(10):847-51. doi: 10.1136/jcp.50.10.847.
4
Determination of penicillin susceptibility of Streptococcus pneumoniae using the polymerase chain reaction.利用聚合酶链反应测定肺炎链球菌对青霉素的敏感性
Mol Pathol. 1997 Feb;50(1):45-50. doi: 10.1136/mp.50.1.45.
5
The enduring pneumococcus: unfinished business and opportunities for the future.持久存在的肺炎球菌：未竟事业与未来机遇
Microb Drug Resist. 1997 Summer;3(2):111-5. doi: 10.1089/mdr.1997.3.111.
6
Combinational detection of autolysin and penicillin-binding protein 2B genes of Streptococcus pneumoniae by PCR.采用聚合酶链反应（PCR）联合检测肺炎链球菌自溶素和青霉素结合蛋白2B基因
J Clin Microbiol. 1996 Mar;34(3):592-6. doi: 10.1128/jcm.34.3.592-596.1996.
7
Detection of Streptococcus pneumoniae in sputum samples by PCR.通过聚合酶链反应（PCR）检测痰液样本中的肺炎链球菌。
J Clin Microbiol. 1994 May;32(5):1308-11. doi: 10.1128/jcm.32.5.1308-1311.1994.
8
Origin and molecular epidemiology of penicillin-binding-protein-mediated resistance to beta-lactam antibiotics.青霉素结合蛋白介导的β-内酰胺类抗生素耐药性的起源与分子流行病学
Trends Microbiol. 1994 Oct;2(10):361-6. doi: 10.1016/0966-842x(94)90612-2.
9
A guide to sensitivity testing. Report of the Working Party on Antibiotic Sensitivity Testing of the British Society for Antimicrobial Chemotherapy.
J Antimicrob Chemother. 1991 Jun;27 Suppl D:1-50.
10
Geographic distribution of penicillin-resistant clones of Streptococcus pneumoniae: characterization by penicillin-binding protein profile, surface protein A typing, and multilocus enzyme analysis.
Clin Infect Dis. 1992 Jul;15(1):112-8. doi: 10.1093/clinids/15.1.112.
Zotero 插件