O'Neill A M, Gillespie S H, Whiting G C
Department of Medical Microbiology, Royal Free Hospital School of Medicine, London NW3 2PF, United Kingdom.
J Clin Microbiol. 1999 Jan;37(1):157-60. doi: 10.1128/JCM.37.1.157-160.1999.
A PCR-restriction fragment length polymorphism strategy directed against the pbp2b gene was evaluated for identification of penicillin susceptibility. A total of 106 United Kingdom (U.K.), 30 Danish, and 11 Papua New Guinean strains were tested. Of the U.K. strains, all the susceptible and all but one of the resistant isolates were correctly assigned. By using conventional definitions of "not resistant" and "not susceptible," the sensitivities were 97. 5 and 94.4%, the specificities were 100 and 98.9%, the positive predictive values were 100 and 94.4%, and the negative predictive values were 93.1 and 98.9%, respectively. This technique may allow susceptible (MIC, <0.1 mg/liter) and resistant (MIC, >1 mg/liter) isolates to be distinguished in a single PCR.
针对pbp2b基因的聚合酶链反应-限制性片段长度多态性策略用于评估青霉素敏感性。共检测了106株来自英国、30株来自丹麦以及11株来自巴布亚新几内亚的菌株。在英国菌株中，所有敏感菌株以及除1株外的所有耐药菌株均被正确分类。按照“不耐药”和“不敏感”的传统定义，敏感性分别为97.5%和94.4%，特异性分别为100%和98.9%，阳性预测值分别为100%和94.4%，阴性预测值分别为93.1%和98.9%。该技术可能使敏感（最低抑菌浓度，<0.1毫克/升）和耐药（最低抑菌浓度，>1毫克/升）菌株在一次聚合酶链反应中得以区分。
