Anisimova L A, Suzina N E, Parfenova O V, Boronin A M
Mol Gen Mikrobiol Virusol. 1995 Apr-Jun(2):36-40.
The genes regulating resistance to potassium tellurite were cloned from plasmid pS221 with a broad host range. The studied DNA fragment encodes for the synthesis of two polypeptides with molecular mass of 38 and 43 kD. Metal tellurium crystals were localized in the periplasm of Pseudomonas aeruginosa ML4262 (pBS221) cells by electron microscopy. Results of colony hybridization permit us to propose that the cloned genes of potassium tellurite resistance have sites of homology with plasmids RP4(IncP), R27 (IncH), pBS38, pBS79, and R931(IncP-2).
从具有广泛宿主范围的质粒pS221中克隆出调控对亚碲酸钾抗性的基因。所研究的DNA片段编码合成两种分子量分别为38 kD和43 kD的多肽。通过电子显微镜观察发现，金属碲晶体定位于铜绿假单胞菌ML4262（pBS221）细胞的周质中。菌落杂交结果使我们推测，克隆的亚碲酸钾抗性基因与质粒RP4（IncP）、R27（IncH）、pBS38、pBS79和R931（IncP - 2）具有同源位点。
