不同不相容群质粒中碲酸盐抗性决定因子的发生率。

Incidence of tellurite resistance determinants among plasmids of different incompatibility groups.

作者信息

Hou Y, Taylor D E

机构信息

Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.

出版信息

Plasmid. 1994 Nov;32(3):306-11. doi: 10.1006/plas.1994.1069.

DOI:10.1006/plas.1994.1069

PMID:7899515

Abstract

Twenty tellurite resistance (TeR) plasmids of IncH12, IncH13, and IncHII groups isolated from Enterobacteriaceae hybridized with the pMJ606 (IncHI2) TeR probe, whereas the promiscuous Pseudomonas IncP alpha plasmids hybridized with only the IncP alpha TeR probe (pDT1555). Neither of the TeR probes hybridized with the IncP2 Pseudomonas plasmids, which also specify TeR. None of the plasmids hybridized with pUM3, a plasmid carrying the arsABC genes that encodes resistance to arsenite, arsenate, antimony, and moderate levels of tellurite. These results show that the IncH12 TeR determinant is widespread in the Enterobacteriaceae and support the hypothesis that there are at least five distinct TeR determinants: (1) the telAB genes from the Escherichia coli chromosome; (2) IncHI2; (3) IncP2; (4) arsABC; and (5) the TeR determinants of IncP alpha plasmids.

摘要

从肠杆菌科分离出的20个IncH12、IncH13和IncHII组的抗碲酸盐（TeR）质粒与pMJ606（IncHI2）TeR探针杂交，而混杂的假单胞菌IncPα质粒仅与IncPα TeR探针（pDT1555）杂交。两种TeR探针均未与同样具有TeR特性的IncP2假单胞菌质粒杂交。没有质粒与pUM3杂交，pUM3是一种携带arsABC基因的质粒，该基因编码对亚砷酸盐、砷酸盐、锑和中等水平碲酸盐的抗性。这些结果表明，IncH12 TeR决定簇在肠杆菌科中广泛存在，并支持以下假设：至少有五个不同的TeR决定簇：（1）来自大肠杆菌染色体的telAB基因；（2）IncHI2；（3）IncP2；（4）arsABC；以及（5）IncPα质粒的TeR决定簇。