Djordjević O, Kostić L, Brkić G, Astaldi G
Biomedicine. 1978 Dec;28(6):323-7.
Hyperthermia (42 degrees C) and peptichemio (PTC) applied simultaneously result in an increased killing effect and in a significant induction of single strand breaks (SSB) of DNA molecule. The enhanced killing effect was observed in all circumstances, whenever hyperthermia was applied before, during or after PTC treatment, but the most effective cell killing was obtained when hyperthermia and PTC were applied simultaneously. The results show that PTC concentrations used in these experiments do not induce SSB in DNA molecule during incubation for 2 hours at 37 degrees C. When the cells were exposed to PTC and hyperthermia the induction of SSB was observed. The number of SSB depends on the exposure time and PTC concentration. Cells with a greater number of SSB lost the capacity for repair during post-incubation at 42 degrees C.
同时应用高温（42摄氏度）和化疗药物（PTC）可增强杀伤效果，并显著诱导DNA分子单链断裂（SSB）。无论高温是在PTC治疗之前、期间还是之后应用，在所有情况下均观察到杀伤效果增强，但当高温和PTC同时应用时，细胞杀伤效果最为显著。结果表明，这些实验中使用的PTC浓度在37摄氏度孵育2小时期间不会诱导DNA分子产生SSB。当细胞暴露于PTC和高温时，可观察到SSB的诱导。SSB的数量取决于暴露时间和PTC浓度。在42摄氏度后续孵育期间，具有较多SSB的细胞失去了修复能力。
