van den Berg C, Willemsen V, Hage W, Weisbeek P, Scheres B
Department of Molecular Cell Biology, Utrecht University, The Netherlands.
Nature. 1995 Nov 2;378(6552):62-5. doi: 10.1038/378062a0.
Postembryonic development in plants is achieved by apical meristems. Surgical studies and clonal analysis have revealed indirectly that cells in shoot meristems have no predictable destiny and that position is likely to play a role in the acquisition of cell identity. In contrast to animal systems, there has been no direct evidence for inductive signalling in plants until now. Here we present evidence for such signalling using laser ablation of cells in the root meristem of Arabidopsis thaliana. Although these cells show rigid clonal relationships, we now demonstrate that it is positional control that is most important in the determination of cell fate. Positional signals can be perpetuated from more mature to initial cells to guide the pattern of meristem cell differentiation. This offers an alternative to the general opinion that meristems are the source of patterning information.
植物胚后发育由顶端分生组织完成。外科手术研究和克隆分析间接表明，茎尖分生组织中的细胞没有可预测的命运，位置可能在细胞身份的获得中起作用。与动物系统不同，到目前为止，植物中还没有诱导信号传导的直接证据。在这里，我们利用激光消融拟南芥根分生组织中的细胞，展示了这种信号传导的证据。尽管这些细胞显示出严格的克隆关系，但我们现在证明，在细胞命运的决定中，位置控制是最重要的。位置信号可以从更成熟的细胞传递到起始细胞，以指导分生组织细胞分化的模式。这为分生组织是模式形成信息来源的普遍观点提供了另一种解释。
