Sillevis Smitt P A, Manley G T, Posner J B
Department of Neurology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Neurology. 1995 Oct;45(10):1873-8. doi: 10.1212/wnl.45.10.1873.
Paraneoplastic encephalomyelitis/paraneoplastic sensory neuronopathy (PEM/PSN) associated with small cell lung cancer is characterized by high serum and CSF titers of anti-neuronal (anti-Hu) antibodies and by intrathecal synthesis of anti-Hu IgG. A pathologic role for the anti-Hu antibodies in PEM/PSN is further suggested by reported intraneuronal accumulation of the antibodies in the nervous system of PEM/PSN patients at autopsy. We immunized SJL/J mice, Lewis rats, and Hartley guinea pigs with purified recombinant HuD fusion protein. In spite of high-titer anti-HuD antibodies, neurologic and pathologic examination of the animals was normal. Apparent uptake of purified IgG by neurons in the brain proved to be artifactual.
与小细胞肺癌相关的副肿瘤性脑脊髓炎/副肿瘤性感觉神经元病（PEM/PSN）的特征是血清和脑脊液中抗神经元（抗Hu）抗体滴度高，以及鞘内合成抗Hu IgG。尸检时在PEM/PSN患者的神经系统中发现抗体在神经元内积聚，这进一步提示了抗Hu抗体在PEM/PSN中的病理作用。我们用纯化的重组HuD融合蛋白免疫SJL/J小鼠、Lewis大鼠和Hartley豚鼠。尽管动物产生了高滴度的抗HuD抗体，但对其进行的神经学和病理学检查均正常。大脑中神经元对纯化IgG的明显摄取被证明是人为现象。
