Ishaque M, Kim S J, Kato L
Can J Microbiol. 1978 Dec;24(12):1548-52. doi: 10.1139/m78-247.
Intact cells obtained from Mycobacterium scrofulaceum as well as from mycobacterial strains M.A6 and M.R56 isolated respectively from leprous tissues of armadillo and rat leproma and grown with glycerol as the oxidizable substrate catalyzed complete oxidation of formate. The stoichiometry of formate oxidase system yielded a value of 2 mol of CO2 produced per mole of O2 or per 2 moles of formate consumed. Cell-free preparations from these three strains of mycobacteria contained formate dehydrogenase which was associated exclusively in the particulate fraction. Formate oxidation was markedly stimulated by small amounts of selenite and molybdate added together. Formate-reduced minus oxidized difference spectra disclosed cytochromes of the b type while spectral evidence did not suggest the existence of cytochromes a or c components. The effect of 2-N-heptyl-4-hydroxyquinoline-N-oxide on the redox state of cytochromes indicated that formate oxidation was mediated by cytochrome b with absorption maximum of 556 nm and not of 562 nm.
从瘰疬分枝杆菌以及分别从犰狳麻风组织和大鼠麻风瘤中分离出的分枝杆菌菌株M.A6和M.R56获得的完整细胞，以甘油作为可氧化底物进行培养时，可催化甲酸盐的完全氧化。甲酸盐氧化酶系统的化学计量学表明，每摩尔氧气或每消耗2摩尔甲酸盐可产生2摩尔二氧化碳。这三种分枝杆菌菌株的无细胞制剂含有甲酸盐脱氢酶，该酶仅与颗粒部分相关。同时添加少量亚硒酸盐和钼酸盐可显著刺激甲酸盐氧化。甲酸盐还原减去氧化的差光谱显示存在b型细胞色素，而光谱证据并未表明存在a型或c型细胞色素成分。2-N-庚基-4-羟基喹啉-N-氧化物对细胞色素氧化还原状态的影响表明，甲酸盐氧化是由吸收最大值为556 nm而非562 nm的细胞色素b介导的。
