Andreesen J R, Ljungdahl L G
J Bacteriol. 1973 Nov;116(2):867-73. doi: 10.1128/jb.116.2.867-873.1973.
The formation of the nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase in Clostridium thermoaceticum is stimulated by the presence of molybdate and selenite in the growth medium. The highest formate dehydrogenase activity was obtained with 2.5 x 10(-4) M Na(2)MoO(4) and 5 x 10(-5) Na(2)SeO(3). Tungstate but not vanadate could replace molybdate and stimulate the formation of formate dehydrogenase. Tungstate stimulated activity more than molybdate, and in combination with molybdate the stimulation of formation of formate dehydrogenase was additive. Formate dehydrogenase was isolated from cells grown in the presence of Na(2) (75)SeO(2), and a correlation was observed between bound (75)Se and enzyme activity.
热醋酸梭菌中烟酰胺腺嘌呤二核苷酸磷酸依赖性甲酸脱氢酶的形成受到生长培养基中钼酸盐和亚硒酸盐的刺激。在含有2.5×10⁻⁴ M Na₂MoO₄和5×10⁻⁵ Na₂SeO₃的条件下可获得最高的甲酸脱氢酶活性。钨酸盐而非钒酸盐能够替代钼酸盐并刺激甲酸脱氢酶的形成。钨酸盐比钼酸盐更能刺激酶活性，并且与钼酸盐联合使用时，对甲酸脱氢酶形成的刺激作用具有加和性。从在Na₂(⁷⁵)SeO₂存在下生长的细胞中分离出甲酸脱氢酶，并且观察到结合的(⁷⁵)Se与酶活性之间存在相关性。
