Segal M
Department of Neurobiology, Weizmann Institute, Rehovot, Israel.
Neurosci Lett. 1995 Jun 30;193(2):73-6. doi: 10.1016/0304-3940(95)11665-j.
Individual cultured rat hippocampal neurons grown on glass coverslips were loaded with lucifer yellow (LY) and segments of their dendrites, containing dendritic spines, were exposed to the glutamate agonist N-methyl-D-aspartate (NMDA). Repeated pulse application of NMDA caused a reduction of spine length by 20%, within 1-2 h of exposure to the drug. No such changes were seen in spines exposed to NMDA in presence of the antagonist 2-aminophosphonovalerate or in spines on sister dendrites which were not exposed to NMDA. It is suggested that dendritic spines shrink following exposure to excessive synaptic stimulation.
将生长在玻璃盖玻片上的单个培养大鼠海马神经元用荧光黄（LY）进行标记，并将其包含树突棘的树突段暴露于谷氨酸激动剂N-甲基-D-天冬氨酸（NMDA）中。在接触该药物的1-2小时内，重复脉冲施加NMDA导致棘突长度减少20%。在存在拮抗剂2-氨基膦酰戊酸的情况下暴露于NMDA的棘突或未暴露于NMDA的姐妹树突上的棘突中未观察到此类变化。这表明树突棘在暴露于过度的突触刺激后会收缩。
