Ikeda K, Shirao T, Toda M, Asada H, Toya S, Uyemura K
Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
Neurosci Lett. 1995 Jul 21;194(3):197-200. doi: 10.1016/0304-3940(95)11760-t.
Drebrin A expression was induced in non-neuronal L cells via transfection with a vector containing the cDNA of rat drebrin A. Following treatment with colcemid (5 micrograms/ml) and cytochalasin D (0.5 micrograms/ml), most L cells collapsed into round cells, while drebrin expressing cells were resistant to the treatment, keeping their cell shapes. Simultaneously, actin filaments and microtubules were disrupted in both cell lines. By quantitative analysis, in the presence of cytochalasin D, the extent of cell spreading and cell attachment in drebrin expressing cells was significantly higher than that in control cells. These results suggest that drebrin A modulates cell-substratum adhesion.
通过用含有大鼠drebrin A cDNA的载体转染，在非神经元L细胞中诱导出drebrin A表达。用秋水仙酰胺（5微克/毫升）和细胞松弛素D（0.5微克/毫升）处理后，大多数L细胞塌陷成圆形细胞，而表达drebrin的细胞对该处理具有抗性，保持其细胞形状。同时，两种细胞系中的肌动蛋白丝和微管均被破坏。通过定量分析，在细胞松弛素D存在的情况下，表达drebrin的细胞中细胞铺展和细胞附着的程度明显高于对照细胞。这些结果表明drebrin A调节细胞与基质的粘附。
