Moldvay J, Deny P, Pol S, Brechot C, Lamas E
INSERM U 370, CHU Necker, Párizs, Franciaország.
Orv Hetil. 1995 Oct 15;136(42):2267-71.
In situ hybridization was used to investigate hepatitis C virus infection in peripheral blood mononuclear cells in 11 patients with chronic hepatitis. Using 35S labeled HCV-RNA probe, HCV-RNA positive and negative strands were observed in unstimulated cells of 3/11 patients; all 3 being treated with immunosuppressive drugs after orthotopic liver transplantation. HCV-RNA sequences were also identified in mononuclear cells obtained from 3 patients without immunosuppression, after stimulation of the cells with either phytohemagglutinin or pokeweed mitogen. In contrast, HCV-RNA was not found in 5 cases, where there was no liver transplantation or cell stimulation by mitogens. These results definitely assess infection of mononuclear cells by HCV. In addition, they demonstrate that mitogenic stimulation of infected cells increases HCV-RNA replication.
采用原位杂交法对11例慢性肝炎患者外周血单个核细胞中的丙型肝炎病毒感染情况进行了研究。使用35S标记的HCV-RNA探针，在11例患者中，有3例未经刺激的细胞中观察到HCV-RNA正链和负链；这3例患者均在原位肝移植后接受免疫抑制药物治疗。在用植物血凝素或商陆有丝分裂原刺激细胞后，在3例未接受免疫抑制的患者的单个核细胞中也鉴定出了HCV-RNA序列。相比之下，在5例未进行肝移植或未用丝裂原刺激细胞的患者中未发现HCV-RNA。这些结果明确评估了HCV对单个核细胞的感染情况。此外，它们还表明，对感染细胞的有丝分裂原刺激会增加HCV-RNA的复制。
