Moldvay J, Deny P, Pol S, Brechot C, Lamas E
INSERM U-370, CHU Necker, Paris, France.
Blood. 1994 Jan 1;83(1):269-73.
We used in situ hybridization to detect hepatitis C virus (HCV) infection of peripheral blood mononuclear cells (PBMNC) from 11 patients with chronic active hepatitis. Using 35S-labeled HCV-RNA probe, HCV-RNA-positive and -negative strands were observed in unstimulated PBMNC from three patients, all of whom were receiving immunosuppressive drugs after orthotopic liver transplantation (OLT). HCV-RNA sequences were also identified in PBMNC from three patients who were not undergoing immunosuppression, after stimulation with either phytohemagglutinin (PHA) or pokeweed mitogen (PWM). In contrast, HCV-RNA was not found in the remaining five patients, who had not undergone OLT and whose cells were not stimulated with mitogens. These results show that mononuclear cells can be infected by HCV and that mitogenic stimulation of infected cells increases HCV-RNA replication.
我们采用原位杂交技术检测了11例慢性活动性肝炎患者外周血单个核细胞（PBMNC）中的丙型肝炎病毒（HCV）感染情况。使用35S标记的HCV - RNA探针，在3例患者未经刺激的PBMNC中观察到HCV - RNA阳性和阴性链，这3例患者均在原位肝移植（OLT）后接受免疫抑制药物治疗。在用植物血凝素（PHA）或商陆有丝分裂原（PWM）刺激后，在3例未接受免疫抑制治疗患者的PBMNC中也鉴定出HCV - RNA序列。相比之下，在其余5例未接受OLT且细胞未用有丝分裂原刺激的患者中未发现HCV - RNA。这些结果表明单核细胞可被HCV感染，并且对感染细胞的有丝分裂原刺激可增加HCV - RNA复制。
