Uzawa N, Yoshida M A, Oshimura M, Ikeuchi T
Department of Cytogenetics, Medical Research Institute, Tokyo, Japan.
Oncogene. 1995 Nov 16;11(10):1997-2004.
It has been suggested that loss of the short arm of chromosome 3 is one of the most frequent abnormalities in human head and neck cancers including oral squamous cell carcinomas (SCC) and that one or more putative tumor suppressor gene(s) which may contribute to the initiation and/or progression of these tumors might be located on chromosome 3p. In this study, we examined the effects of introducing human chromosome 3 or 7 by microcell hybridization on the tumor-associated phenotypes of three different human oral SCC cell lines, HSC-2, HSC-3 and HSC-4. Transfer of a single chromosome 3p completely suppressed the tumorigenicity of all three parental cell lines, which showed a significant decrease in growth rate in vitro and morphological changes. In contrast, transfer of chromosome 7 had no effect on HSC-2 and HSC-4 cells, although it suppressed the tumorigenicity of HSC-3 cells without modifying their in vitro growth properties. Our findings provide additional confirmatory evidence that loss or inactivation of putative tumor suppressor gene(s) present on chromosome 3p might be primarily involved in the development of human oral SCC. The possibility that chromosome 7 may carry another tumor suppressor gene(s) is also discussed.
有人提出，3号染色体短臂缺失是包括口腔鳞状细胞癌（SCC）在内的人类头颈癌中最常见的异常之一，并且一个或多个可能促成这些肿瘤发生和/或进展的假定肿瘤抑制基因可能位于3号染色体短臂上。在本研究中，我们通过微细胞杂交引入人类3号或7号染色体，检测其对三种不同的人类口腔SCC细胞系HSC - 2、HSC - 3和HSC - 4的肿瘤相关表型的影响。单个3号染色体短臂的转移完全抑制了所有三个亲代细胞系的致瘤性，这些细胞系在体外的生长速率显著降低且形态发生改变。相比之下，7号染色体的转移对HSC - 2和HSC - 4细胞没有影响，尽管它抑制了HSC - 3细胞的致瘤性，但未改变其体外生长特性。我们的研究结果提供了额外的确证证据，表明3号染色体短臂上存在的假定肿瘤抑制基因的缺失或失活可能主要参与了人类口腔SCC的发生发展。同时也讨论了7号染色体可能携带另一种肿瘤抑制基因的可能性。
